Multidrug resistant lncRNA profile in chemotherapeutic sensitive and resistant ovarian cancer cells.
Multidrug resistant lncRNA profile in chemotherapeutic sensitive and resistant ovarian cancer cells.
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化疗敏感和耐药卵巢癌细胞中的多药耐药 lncRNA 谱。
DOI：
10.1002/jcp.26369
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
通讯作者）
中科院分区：
文献类型：
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作者：
Juan Xu;Jiacong Wu;Chenyang Fu;Fang Teng;Siyu Liu;Chencheng Dai;Rong Shen;Xuemei Jia（贾雪梅;通讯作者）
大多数卵巢癌患者最初是化疗敏感的，但最终复发，获得性耐药。多药耐药是一种极其可怕的情况。卵巢癌患者获得性化疗耐药的机制尚不清楚。LncRNA被认为是多种生物过程的重要调节因子，包括多药耐药过程。我们对卵巢癌细胞株A2780和对顺铂和表阿霉素交叉耐药的紫杉醇耐药细胞株A2780/PTX进行了lncRNA测序。通过整合已发表的数据与卵巢癌细胞系或卵巢癌患者中顺铂耐药的lncRNA，5种上调和21种下调的lncRNA被认为是多药耐药的lncRNA。通过真实的实时PCR分析，我们证实了5个上调和4个下调的多药耐药lncRNA在多药耐药卵巢癌细胞系和多药耐药结肠癌细胞系中的变化相似。此外，我们进行了lncRNA-mRNA共表达网络，以预测潜在的多药耐药lncRNA的靶点。有趣的是，多药耐药基因ABCB 1、ABCB 4、ABCC 3和ABCG 2都与lncRNA CTD-2589M5.4共表达。本研究结果为进一步了解lncRNA在多药耐药过程中的作用提供了有价值的信息。
Most ovarian cancer patients are chemosensitive initially, but finally relapse with acquired chemoresistance. Multidrug‐resistance is the extremely terrible situation. The mechanism for the acquired chemoresistance of ovarian cancer patients is still not clear. LncRNAs have been recognized as the important regulator of a variety of biological processes, including the multidrug‐resistant process. Here, we carried out the lncRNA sequencing of the ovarian cancer cell line A2780 and the paxitaxel resistant cell line A2780/PTX which is also cross resistant to the cisplatin and epirubicin. Through integrating the published data with the cisplatin resistant lncRNAs in ovarian cancer cell line or ovarian cancer patients, 5 up‐regulated and 21 down‐regulated lncRNAs are considered as the multidrug‐resistant lncRNAs. By real‐time PCR analysis, we confirmed the 5 up‐regulated and 4 down‐regulated multidrug resistant lncRNAs were similarly changed in both the multidrug resistant ovarian cancer cell lines and the multidrug resistant colon cancer cell lines. Furthermore, we conducted the lncRNA‐mRNA co‐expression network to predict the potential multidrug resistant lncRNAs’ targets. Interestingly, the multidrug resistant genes ABCB1, ABCB4, ABCC3, and ABCG2 are all co‐expressed with lncRNA CTD‐2589M5.4. Our results provide the valuable information for the understanding of the lncRNA function in the multidrug resistant process.