Identification of differentially activated pathways in Phytophthora sojae at the mycelial, cyst, and oospore stages by TMT-based quantitative proteomics analysis
Identification of differentially activated pathways in Phytophthora sojae at the mycelial, cyst, and oospore stages by TMT-based quantitative proteomics analysis
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基于 TMT 的定量蛋白质组学分析鉴定酱油疫霉菌丝体、胞囊和卵孢子阶段的差异激活途径
DOI：
10.1016/j.jprot.2020.103776
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发表时间：
2020
影响因子：
3.3
通讯作者：
Xili Liu
中科院分区：
文献类型：
--
作者：
Can Zhang;Tongshan Cui;Fan Zhang;Zhaolin Xue;Jianqiang Miao;Weizhen Wang;Xili Liu
植腐菌属（Phytophthorasojae）是一种分布广泛、具有破坏性的卵菌植物病原菌，已被开发为卵菌生物学的模式。鉴于疫霉有性阶段和无性阶段比较的重要但有限的报道，我们对大豆疫霉的两个关键无性生活阶段-菌丝体和孢囊-以及卵孢子进行了大规模的定量蛋白质组学研究，这是一个关键的有性阶段。分析了来自4688种蛋白质的超过29，631种肽。简言之，分别将445种蛋白质、624种蛋白质和579种蛋白质定义为孢囊与菌丝体、卵孢子与孢囊和卵孢子与菌丝体比较中的差异定量蛋白质（|log2倍数变化|> 1，P <0.05）。与菌丝体和卵孢子相比，孢囊中脂肪酸和氮代谢被特异性地诱导。在卵孢子中，上调的蛋白质集中在内质网中的RNA运输和蛋白质加工，表明翻译，折叠和分泌的核心细胞或阶段特异性蛋白质在卵孢子中的活性，这可能是用于卵孢子萌发。这些数据扩大了我们对这种病原体的无性和有性阶段的途径的认识。生物学意义卵菌中的有性孢子（卵孢子）具有厚的细胞壁，可以在土壤中存活多年，从而提供了一个主要来源，并允许在随后的生长季节重新感染宿主植物。然而，卵孢子的蛋白质组学研究仍然非常有限，因为它们通常被认为是休眠的。本研究成功分离了大豆疫霉卵孢子，并对该关键有性阶段和两个代表性无性阶段进行了大规模的比较定量蛋白质组学研究。这些结果提供了对大豆疫霉生物学的进一步理解，并为卵菌三个不同发育阶段的疾病控制提供了潜在的代谢靶点。
Phytophthorasojaeis a widely distributed, destructive oomycete plant pathogen that has been developed as a model for oomycete biology. Given the important but limited reports on the comparison of the sexual and asexual stages inPhytophthora, we performed a large-scale quantitative proteomics study on two key asexual life stages ofP. sojae—the mycelium and cyst—as well as on the oospore, which is a key sexual stage. Over 29,631 peptides from 4688 proteins were analyzed. Briefly, 445 proteins, 624 proteins, and 579 proteins were defined as differentially quantified proteins in cyst vs mycelium, oospore vs cyst, and oospore vs mycelium comparisons, respectively (|log2 fold change| > 1 andP< 0.05). Compared to the mycelium and oospore, fatty acid and nitrogen metabolism were specifically induced in cysts. In oospores, the up-regulated proteins focused on RNA transport and protein processing in endoplasmic reticulum, indicating translation, folding, and the secretion of core cellular or stage-specific proteins active in oospores, which might be used for oospore germination. The data presented expand our knowledge of pathways specifically linked to asexual and sexual stages of this pathogen.Biological significanceThe sexual spores (oospores) in oomycetes have thick cell walls and can survive in the soil for years, thus providing a primary source and allowing the reinfection of their host plant in subsequent growing seasons. However, the proteomic study on oospores remains very limited as they are generally considered to be dormant. In the present study, we successfully isolated oospores, and performed a large-scale comparative quantitative proteomics study on this key sexual stage and two representative asexual stages ofP. sojae. The results provide an improved understanding ofP. sojaebiology and suggest potential metabolic targets for disease control at the three different developmental stages in oomycetes.