Demonstration that rat prolactin has no intrinsic antidiuretic activity in the rat.
Demonstration that rat prolactin has no intrinsic antidiuretic activity in the rat.
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证明大鼠催乳素在大鼠中没有内在的抗利尿活性。
DOI：
10.1159/000179434
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Adler,RA
中科院分区：
文献类型：
--
作者：
North,WG;Gellai,M;Sokol,HW;Adler,RA
大鼠催乳素具有内在的抗利尿活性的命题进行了研究，使用清醒的大鼠模型（Brattleboro纯合子，DI大鼠），似乎不产生精氨酸加压素（AVP）。从NIAMDD获得的大鼠催乳素制剂（大鼠催乳素B1）在静脉给药这些动物时迅速产生抗利尿作用。用放射免疫分析法测得催乳素B1中AVP含量为150 ng/mg蛋白。用聚丙烯酰胺凝胶电泳从催乳素B1中除去所有可测量的AVP，并且除去的催乳素完全没有任何抗利尿活性。添加合成的AVP，以相当于AVP污染的催乳素B1的量，完全恢复了最初观察到的抗利尿活性。单独给予合成AVP产生类似的抗利尿作用，但在存在氢化催乳素的情况下，这些作用是由更少量的AVP产生的。我们认为，我们的数据表明，大鼠催乳素没有内在的抗利尿活性，并与不同的催乳素制剂的抗利尿活性可能完全是由于他们的污染与AVP。数据还表明，催乳素的存在增强了约5倍的AVP的抗利尿活性。
The proposition that rat prolactin has an intrinsic antidiuretic activity was examined using a conscious rat model (Brattleboro homozygotes, DI rats) which does not appear to produce arginine vasopressin (AVP). A preparation of rat prolactin obtained from NIAMDD, rat prolactin Bl, produced a prompt antidiuresis when administered intravenously to these animals. Prolactin Bl was found to contain 150ng of AVP/mg protein by RIA. All of the measurable AVP was removed from prolactin Bl by polyacrylamide gel electrophoresis and the electrophoresed prolactin was completely devoid of any antidiuretic activity. Addition of synthetic AVP to electrophoresed prolactin in an amount equivalent to the AVP contamination of prolactin B1 completely restored the originally observed antidiuretic activity. Synthetic AVP given alone produced a similar antidiuresis, but in the presence of electrophoresed prolactin these effects were produced by much smaller amounts of AVP. We believe that our data demonstrate that rat prolactin has no intrinsic antidiuretic activity, and that the antidiuretic activity associated with different prolactin preparations could be entirely due to their contamination with AVP. The data also show that the presence of prolactin enhanced by about five times the antidiuretic activity of AVP.