The orientation of a decellularized uterine scaffold determines the tissue topology and architecture of the regenerated uterus in rats
The orientation of a decellularized uterine scaffold determines the tissue topology and architecture of the regenerated uterus in rats
复制标题
DOI：
10.1093/biolre/ioz004
复制
发表时间：
2019-05-01
影响因子：
3.6
通讯作者：
Tanaka, Mamoru
中科院分区：
文献类型：
--
作者：
Miki, Fumie;Maruyama, Tetsuo;Tanaka, Mamoru
从大鼠制备的脱细胞子宫支架（DUS）当放置在部分切除的子宫上时允许子宫组织的再细胞化和再生，并且以与完整子宫相当的方式支持妊娠。在DUS中保存的基础细胞外基质（ECM）和无细胞的可灌注血管结构被认为是子宫适当再生的原因。为了研究这一概念，我们通过将DUS以相反的方向放置在部分缺陷的子宫区域上，使DUS的腔面在外面，检查了保留DUS的ECM的方向和血管结构对子宫再生的影响。浆膜面在里面。我们描述了再生子宫的组织结构和功能，并将结果与DUS放置在正确方向时的结果进行了比较。组织学分析显示，逆转组的异常结构，包括腺体异位定位和平滑肌层异常衬里，明显多于正确组（70%比30%，P
A decellularized uterine scaffold (DUS) prepared from rats permits recellularization and regeneration of uterine tissues when placed onto a partially excised uterus and supports pregnancy in a fashion comparable to the intact uterus. The underlying extracellular matrix (ECM) together with an acellular, perfusable vascular architecture preserved in DUS is thought to be responsible for appropriate regeneration of the uterus. To investigate this concept, we examined the effect of the orientation of the DUS-preserving ECM and the vascular architecture on uterine regeneration through placement of a DUS onto a partially defective uterine area in the reversed orientation such that the luminal face of the DUS was outside and the serosal face was inside. We characterized the tissue structure and function of the regenerated uterus, comparing the outcome to that when the DUS was placed in the correct orientation. Histological analysis revealed that aberrant structures including ectopic location of glands and an abnormal lining of smooth muscle layers were observed significantly more frequently in the reversed group than in the correct group (70% vs. 30%, P