Chemotactic cytokines: the role of leukocytic pyrogen and epidermal cell thymocyte-activating factor in neutrophil chemotaxis.
Chemotactic cytokines: the role of leukocytic pyrogen and epidermal cell thymocyte-activating factor in neutrophil chemotaxis.
复制标题
趋化细胞因子：白细胞热原和表皮细胞胸腺细胞激活因子在中性粒细胞趋化中的作用。
作者：
D. Sauder;N. Mounessa;S. Katz;C. Dinarello;J. Gallin
巨噬细胞衍生的白细胞热原 (LP) 被认为与白细胞介素 1 (IL 1) 相似，即使不相同。除了巨噬细胞外，角质形成细胞还产生一种与 IL-1 具有相似生物学和生化特性的因子，称为表皮细胞胸腺细胞激活因子 (ETAF)。由于许多影响皮肤的疾病都以多形核白细胞 (PMN) 浸润为特征，并且其中一些疾病与发烧有关，因此我们研究了 ETAF 与 LP 是否具有致热活性，以及 ETAF 或 LP 是否是化学引诱剂。通过柱层析和等电聚焦纯化的 ETAF 或 LP 被发现对 PMN 具有趋化活性。含有最大趋化活性的级分对应于最大发热诱导活性和最大胸腺细胞激活活性。此外，ETAF 和 LP 的趋化活性可以被针对 LP 的抗体阻断。这项研究的结果表明，这些由不同细胞群产生的介质密切相关，可能在皮肤以及远处炎症和免疫事件中发挥重要作用。
Macrophage-derived leukocytic pyrogen (LP) is thought to be similar if not identical to interleukin 1 (IL 1). In addition to macrophages, keratinocytes produce a factor that has similar biologic and biochemical characteristics to IL 1, called epidermal cell thymocyte-activating factor (ETAF). Because many diseases affecting the skin are characterized by infiltration of polymorphonuclear leukocytes (PMN) and some of these disorders are associated with fever, we investigated whether ETAF like LP had pyrogenic activity, and whether ETAF or LP were chemoattractants. ETAF or LP purified by column chromatography and isoelectric focusing were found to have chemotactic activity for PMN. The fractions containing maximal chemotactic activity corresponded to maximal fever-inducing activity and maximum thymocyte-activating activity. Furthermore, the chemotactic activity of ETAF and LP could be blocked by an antibody directed against LP. The results of this study indicate that these mediators, which arise from distinct cell populations, are closely related and may play a vital role in skin as well as distant inflammatory and immunologic events.