Enzyme responsive titanium substrates with antibacterial property and osteo/angio-genic differentiation potentials
Enzyme responsive titanium substrates with antibacterial property and osteo/angio-genic differentiation potentials
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具有抗菌特性和骨/血管生成分化潜力的酶响应钛基质
DOI：
10.1016/j.colsurfb.2019.110592
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Cai Kaiyong
中科院分区：
文献类型：
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作者：
Yu Yonglin;Ran Qichun;Shen Xinkun;Zheng Hong;Cai Kaiyong
钛（Ti）骨科材料植入宿主后面临着细菌感染和无菌性松动两大临床挑战，这直接决定了植入物的长期存活。为了赋予钛植入物自卫抗菌特性和理想的骨/血管生成分化潜力，通过逐层（LBL）组装技术，在负载去铁胺（DFO）的二氧化钛纳米管（TNT）基底上构建透明质酸（HA）-庆大霉素（Gen）缀合物（HA-Gen）和壳聚糖（Chi）聚电解质多层膜，称为TNT/DFO/HA-Gen。 HA-Gen 缀合物通过傅里叶变换红外光谱 (FTIR) 和核磁共振 (1H NMR) 进行表征。通过场发射扫描电子显微镜（FE-SEM）、原子力显微镜（AFM）、X射线光电子能谱（XPS）和接触角测量来表征基材的物理化学性质。按需 DFO 释放与外源透明质酸酶引发的多层降解有关，这表明酶促和细菌响应。 TNT/DFO/HA-Gen基质对大肠杆菌(E. coli)和金黄色葡萄球菌(S. aureus)表现出有效的防污和抗菌性能，同时有利于间充质干细胞(MSC)的粘附、增殖和成骨/血管生成分化。多方面的药物器械组合（DDC）策略显示出在骨科领域的潜在应用。
After implantation into a host, titanium (Ti) orthopaedic materials are facing two major clinical challenges: bacterial infection and aseptic loosening, which directly determine the long-term survival of the implant. To endow Ti implant with self-defensive antibacterial properties and desirable osteo/angio-genic differentiation potentials, hyaluronic acid (HA)-gentamicin (Gen) conjugates (HA-Gen) and chitosan (Chi) polyelectrolyte multilayers were constructed on deferoxamine (DFO) loaded titania nanotubes (TNT) substratesvialayer-by-layer (LBL) assembly technique, termed as TNT/DFO/HA-Gen. The HA-Gen conjugate was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H NMR). The physicochemical properties of the substrates were characterized by field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The on-demand DFO release was associated with the degradation of multilayers triggered by exogenous hyaluronidase, which indicated enzymatic and bacterial responsiveness. The TNT/DFO/HA-Gen substrates displayed effective antifouling and antibacterial properties againstEscherichia coli(E. coli) andStaphylococcus aureus(S. aureus), while were favourable for the adhesion, proliferation and osteo/angio-genic differentiation of mesenchymal stem cells (MSCs). The multifaceted drug-device combination (DDC) strategy showed potential applications in orthopaedic fields.