A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads.
A novel method for large-scale immuno-SEM using protein G coupled polystyrene beads.
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一种使用蛋白 G 偶联聚苯乙烯珠进行大规模免疫 SEM 的新方法。
DOI：
10.1093/jmicro/dfq064
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Dennis,JamesE
中科院分区：
文献类型：
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作者：
Song,In-Hwan;Dennis,JamesE
将平均直径为 0.76 μm 的聚苯乙烯珠与蛋白 G 偶联，然后将抗 II 型胶原蛋白 IgG 或抗 4-硫酸软骨素 IgG 标记到蛋白 G 上。将带有抗体标记的珠应用于关节软骨，并对每个样品中标记的珠进行计数。抗体标记的珠子标记显着高于 IgG 同型对照。我们建议使用蛋白 G 偶联珠的免疫扫描电镜作为一种有价值的方法，用于观察组织或器官表面特定蛋白质分布的微米范围。这将同时提供有关表面结构和抗原性的信息。
Polystyrene beads with a mean diameter of 0.76 μm were coupled with protein G and then anti-type II collagen IgG or anti-chondroitin-4-sulphate IgG were tagged to protein G. Antibody-tagged beads were applied to articular cartilage and labelled beads were counted in each sample. Antibody-tagged beads labelled significantly higher than IgG isotype control. We propose immuno-SEM using protein G coupled beads as a valuable method for micrometre range observation for specific protein distribution on surfaces of tissues or organs. This will provide information about structure as well as antigenicity on the surface at the same time.