Effect of Matrix Metalloproteinase on Autolysis of Sea Cucumber Stichopus japonicus
Effect of Matrix Metalloproteinase on Autolysis of Sea Cucumber Stichopus japonicus
复制标题
基质金属蛋白酶对海参自溶作用的影响
DOI：
10.1007/s10068-013-0210-x
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发表时间：
2013-10-01
影响因子：
2.9
通讯作者：
Murata, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Sun, Li-Ming;Wang, Ting-Ting;Murata, Yoshiyuki
为了研究基质金属蛋白酶（MMPs）与日本参（Stichopus japonicus）自溶的关系，将日本参真皮匀浆在25℃下培养，诱导其自溶。用EDTA Na-2和1,10-菲罗啉检测MMPs对自溶的影响，采用可溶性蛋白和蛋白谱法测定。可溶性蛋白水平在6小时的自溶过程中升高。SDS-PAGE显示出明显的蛋白质降解，并伴有降解产物的出现。EDTA Na-2和1,10-菲罗啉均能显著抑制上述两项指标，提示MMPs可能在海参自溶过程中发挥重要作用。
To investigate the relationship between matrix metalloproteinase (MMPs) and autolysis of sea cucumber Stichopus japonicus, the dermis homogenate was incubated at 25 degrees C to induce autolysis. EDTA Na-2 and 1,10-phenanthroline were used to verify the effect of MMPs on autolysis, which was measured by soluble protein and protein pattern. Soluble protein level increased during a 6-h autolysis process. SDS-PAGE demonstrated obvious protein degradation with the concomitant occurrence of degradation products. The above two indicators could be inhibited significantly by EDTA Na-2 and 1,10-phenanthroline, indicating that MMPs might play a significant role in autolysis of sea cucumber.