Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes
Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes
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DOI：
10.1104/pp.17.01035
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发表时间：
2017-11-01
期刊：
影响因子：
7.4
通讯作者：
Sugimoto, Keiko
中科院分区：
文献类型：
--
作者：
Ikeuchi, Momoko;Iwase, Akira;Sugimoto, Keiko
创伤是器官再生的主要触发因素，但创伤应激如何重新激活细胞增殖并促进细胞重编程仍然是一个谜。在这项研究中，我们结合转录组分析和定量激素分析，以探讨如何创伤诱导愈伤组织形成在拟南芥（拟南芥）。我们的时间过程RNA-seq分析表明，创伤诱导动态转录变化，从快速应激反应开始，随后激活代谢过程和蛋白质合成，随后激活细胞周期调节因子。基因本体分析进一步揭示了创伤改变了激素生物合成和响应基因的表达，并且内源植物激素的定量分析揭示了在愈伤组织形成之前细胞分裂素的积累。细胞分裂素合成和信号传导缺陷的突变体显示出愈伤组织形成的效率降低，表明细胞分裂素的从头合成对于伤口诱导的愈伤组织形成至关重要。我们进一步证明，B型拟南芥反应调节因子介导的细胞分裂素信号调节细胞周期蛋白D3;1（CYCD 3;1）的表达，CYCD 3;1及其同源物CYCD 3;2和3的突变导致愈伤组织形成缺陷。除了这些创伤介导的变化，我们的转录组数据揭示了创伤激活多种发育调节因子，我们发现了乙烯反应因子115和PLETHORA 3（PLT 3），PLT 5和PLT 7在愈伤组织生成中的新作用。总之，这些结果提供了新的机制的见解如何创伤愈合过程中重新激活细胞增殖。
Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.