Paper based colorimetric biosensing platform utilizing cross-linked siloxane as probe.
Paper based colorimetric biosensing platform utilizing cross-linked siloxane as probe.
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DOI：
10.1016/j.bios.2013.11.065
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发表时间：
2014-05
影响因子：
12.6
通讯作者：
Miao Zhou;Minghui Yang;F. Zhou
中科院分区：
文献类型：
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作者：
Miao Zhou;Minghui Yang;F. Zhou
开发了利用交联硅氧烷 3-氨丙基三乙氧基硅烷 (APTMS) 作为探针的纸基比色生物传感平台，用于检测包括 H2O2、葡萄糖和蛋白质生物标志物在内的广泛目标。 APTMS 被广泛用于滤纸的改性以开发纸基分析设备。我们发现，当 APTMS 与戊二醛 (GA) 交联时，所得复合物 (APTMS-GA) 显示砖红色，并且当复合物与 H2O2 反应时观察到视觉颜色变化。通过将APTMS-GA复合物与滤纸集成，改性纸可以通过软件Image J监测纸的颜色强度变化来定量检测H2O2。然后，通过将葡萄糖氧化酶（GOx）固定在改性纸上，可以通过检测酶促产生的H2O2来检测葡萄糖。对于蛋白质生物标志物前列腺特异性抗原 (PSA) 测定，我们将捕获、未捕获的抗 PSA 抗体 (Ab1) 固定到纸表面，并使用 GOx 修饰的金纳米棒 (GNR) 作为检测抗 PSA 抗体 (Ab2) 标记。当 GOx 标记与葡萄糖反应时，释放出的 H2O2 也可实现 PSA 的检测。结果证明了基于本文的传感器用于检测具有宽线性范围的不同分析物的可能性。这种纸质传感器成本低且简单，可用于“现场护理”分析，并在不同领域得到广泛应用。
Paper based colorimetric biosensing platform utilizing cross-linked siloxane 3-aminopropyltriethoxysilane (APTMS) as probe was developed for the detection of a broad range of targets including H2O2, glucose and protein biomarker. APTMS was extensively used for the modification of filter papers to develop paper based analytical devices. We discovered when APTMS was cross-linked with glutaraldehyde (GA), the resulting complex (APTMS–GA) displays brick-red color, and a visual color change was observed when the complex reacted with H2O2. By integrating the APTMS–GA complex with filter paper, the modified paper enables quantitative detection of H2O2through the monitoring of the color intensity change of the paper via software Image J. Then, with the immobilization of glucose oxidase (GOx) onto the modified paper, glucose can be detected through the detection of enzymatically generated H2O2. For protein biomarker prostate specific antigen (PSA) assay, we immobilized capture, not captured anti-PSA antibody (Ab1) onto the paper surface and using GOx modified gold nanorod (GNR) as detection anti-PSA antibody (Ab2) label. The detection of PSA was also achieved via the liberated H2O2when the GOx label reacted with glucose. The results demonstrated the possibility of this paper based sensor for the detection of different analytes with wide linear range. The low cost and simplicity of this paper based sensor could be developed for “point-of-care” analysis and find wide application in different areas.