Plasma 8-isoprostane is increased in preterm infants who develop bronchopulmonary dysplasia or periventricular leukomalacia
Plasma 8-isoprostane is increased in preterm infants who develop bronchopulmonary dysplasia or periventricular leukomalacia
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DOI：
10.1203/01.pdr.0000130478.05324.9d
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发表时间：
2004-07-01
影响因子：
3.6
通讯作者：
Lapatto, R
中科院分区：
文献类型：
--
作者：
Ahola, T;Fellman, V;Lapatto, R
我们的目的是评估血浆游离8-表-前列腺素F-2 α（8-异前列腺素）和抗坏血酸自由基作为极低出生体重儿（ELBWI）氧化损伤的风险指标，以及N-乙酰半胱氨酸（NAC）对这些标志物的影响。在出生后第3天和第7天从入组随机对照试验的婴儿中采集血浆样本，在该试验中，在出生后第一周内对ELBWI静脉注射NAC或安慰剂，目的是预防支气管肺发育不良（BPD）。采用酶免疫试剂盒分析了83例婴儿的血浆8-异前列烷。本文用电子自旋共振波谱法测定了61例婴幼儿血清抗坏血酸自由基浓度。NAC组和安慰剂组的8-异前列烷浓度相似。在后来发展为BPD或死亡的婴儿中（n = 29），8-异前列烷浓度的中位数（范围）显著高于（p = 0.001）[分别为50.0 pg/mL（19-360）和57.0 pg/mL（14-460）]，而无BPD的存活者[n = 54; 34.5 pg/mL（5-240）和39.5 pg/mL（7-400）]。8-异前列腺素水平在后来发展为脑室周围白质软化的婴儿中显著增加（p < 0.05）。NAC治疗或BPD的后期发展与抗坏血酸自由基水平无关。抗坏血酸自由基水平从第3天到第7天在所有组中均显著降低，但组间差异不显著。第3天平均抗坏血酸自由基水平在后来发展为脑室周围白质软化的婴儿中显著较高（p < 0.01）[287（124）vs 194（90）]。这些数据表明，血浆8-异前列烷可以作为评估ELBWI发生BPD风险的标志物。
Our aim was to assess the plasma free 8-epi-prostaglandin F-2alpha (8-isoprostane) and ascorbyl radical as risk indicators for oxidative damage in extremely low birth weight infants (ELBWIs) and the effect of N-acetylcysteine (NAC) on these markers. Plasma samples were collected on days 3 and 7 of life from infants who were enrolled in a randomized, controlled trial in which i.v. NAC or placebo was administered to ELBWIs during the first week of life, with the aim of preventing bronchopulmonary dysplasia (BPD). Plasma 8-isoprostane was analyzed in 83 infants using an enzyme immunoassay kit. Ascorbyl radical concentration was measured in 61 infants with electron spin resonance spectroscopy. The 8-isoprostane concentrations were similar in the NAC and placebo groups. In infants who later developed BPD or died (n = 29), the median (range) 8-isoprostane concentration was significantly higher (p = 0.001) on day 3 and day 7 [50.0 pg/mL (19-360) and 57.0 pg/mL (14-460), respectively] than in survivors without BPD [n = 54; 34.5 pg/mL (5-240) and 39.5 pg/mL (7-400), respectively]. The 8-isoprostane levels increased significantly more (p < 0.05) in infants who later developed periventricular leukomalacia. NAC treatment or the later development of BPD was not related to the ascorbyl radical levels. The ascorbyl radical level decreased significantly in all groups from day 3 to day 7, but the difference between the groups was not significant. The mean (SD) ascorbyl radical level on day 3 was significantly higher (p < 0.01) in infants who later developed periventricular leukomalacia [287 (124) versus 194 (90)]. These data suggest that plasma 8-isoprostane could serve as a marker in assessing the risk for BPD development in ELBWIs.