Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides
Rapid construction of fluorescence quenching-based immunosensor Q-bodies using α-helical coiled-coil peptides
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DOI：
10.1039/d1cc02605d
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发表时间：
2021-07-19
影响因子：
4.9
通讯作者：
Ueda, Hiroshi
中科院分区：
文献类型：
--
作者：
Yasuda, Takanobu;Inoue, Akihito;Ueda, Hiroshi
在这里，我们报告了一种快速有效的方法来制造淬灭体（Q-体），可以使用稳定的卷曲螺旋肽对E4和K4（卷曲Q-体，CQ-体）检测抗原依赖性荧光增强的目标。CQ-体允许不仅在缓冲液中而且在50%血浆中进行抗原检测。此外，我们描述了FRET型CQ体使用双色K4肽，这允许更精确的抗原定量。最后，基于纳米抗体的CQ-抗体的成功制造显示了其对一系列抗体片段的适用性。
Here, we report a rapid and efficient method to fabricate Quenchbodies (Q-bodies) that can detect targets with antigen-dependent fluorescence augmentation using a stable coiled-coil peptide pair, E4 and K4 (coiled Q-body, CQ-body). The CQ-body allowed antigen detection not only in buffer but also in 50% plasma. Furthermore, we describe FRET-type CQ-bodies using a dual-coloured K4 peptide, which allowed a more precise antigen quantification. Lastly, successful fabrication of nanobody-based CQ-body shows its applicability to a range of antibody fragments.