Injecting vascular endothelial growth factor into the temporomandibular joint induces osteoarthritis in mice.
Injecting vascular endothelial growth factor into the temporomandibular joint induces osteoarthritis in mice.
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颞下颌关节注射血管内皮生长因子诱导小鼠骨关节炎
DOI：
10.1038/srep16244
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发表时间：
2015-11-04
影响因子：
4.6
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Shen P;Jiao Z;Zheng JS;Xu WF;Zhang SY;Qin A;Yang C
血管内皮生长因子（VEGF）是否能引起颞下颌关节（TMJ）骨关节炎（OA）尚不清楚。本研究观察了关节腔内注射外源性VEGF对小鼠TMJ早期的影响。将48只雄性Sprague-Dawley小鼠等分为3组。在VEGF组中，小鼠在TMJ中接受VEGF溶液（50 μL）的注射，每周一次，持续4周。在假手术组中，小鼠接受生理盐水（50 μL）的注射。对照组不接受任何注射。在第1、2、4和8周处死每组4只小鼠。vegf组观察到逐渐明显的软骨变性。此外，该组显示出较高的金属蛋白酶（MMP）-9，MMP-13，核因子-κ-B配体受体激活剂（RANKL）表达以及较高数量的凋亡软骨细胞和VEGF受体2（VEGFR 2）阳性软骨细胞。CT显示VEGF组软骨下骨吸收明显，软骨下骨内破骨细胞增多，体外研究表明VEGF可促进破骨细胞分化。总之，我们的研究发现VEGF可以通过破坏软骨和软骨下骨而引发TMJ OA。
It is unclear whether vascular endothelial growth factor (VEGF) can initiate osteoarthritis (OA) in the temporomandibular joint (TMJ). In this study we evaluated the effects of intra-articular injection of exogenous VEGF in the TMJ in mice on the early stage. Forty-eight male Sprague-Dawley mice were equally divided into 3 groups. In the vegf group, the mice received an injection of VEGF solution (50 μL) in the TMJ once a week over a period of 4 weeks. In the sham group, the mice received an injection of saline (50 μL). The control group did not receive any injection. Four mice from each group were sacrificed at 1, 2, 4 and 8 weeks. Gradual prominent cartilage degeneration was observed in the vegf group. Additionally, this group showed higher expressions of metalloproteinase (MMP)-9, MMP-13, receptor activator of nuclear factor-kappa-B ligand (RANKL) and a higher number of apoptotic chondrocytes and VEGF receptor 2 (VEGFR2)-positive chondrocytes. Micro-computed tomography (CT) revealed prominent subchondral bone resorption in the vegf group, with a high number of osteoclasts in the subchondral bone.In vitrostudy demonstrated that VEGF can promote osteoclast differentiation. In conclusion, our study found that VEGF can initiate TMJ OA by destroying cartilage and subchondral bone.