Simultaneous Determination of Several Phytohormones in Cotton Root by using High Performance Liquid Chromatography
Simultaneous Determination of Several Phytohormones in Cotton Root by using High Performance Liquid Chromatography
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发表时间：
2009
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通讯作者：
Ding Jun-hui
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作者：
Ding Jun-hui
目的：探讨高效液相色谱法同时检测棉花根中几种植物激素含量的方法。方法：玉米素(z)、核糖体玉米素（ZR）、赤霉素（GA3）、生长激素（IAA）、脱落素（ABA）的含量采用反相色谱柱Waters C18(4.6×250mm,5μm), 35℃，以乙腈-三乙胺溶液为流动相，流速1mL梯度洗脱。Min-1，每种物质的检测波长取决于其在保留时间内的最大吸收峰波长。结果：5种激素的灵敏度和回收率均较高，其中z96.82%、ZR 94.14%、GA3 92.75%、IAA 93.38%、ABA 95.57%。5种植物激素的检出限均较低，ZR为0.1μg.mL-1，ZR为0.1μg.mL-1，GA3为0.5μg.mL-1，IAA为0.3 μg. ml -1，ABA为0.05μg.mL-1。可以准确检测出5种植物激素（Z、ZR、GA3、IAA和ABA）的含量。结论：建立了利用波长变化的高效液相色谱法同时检测植物组织中几种激素含量的方法。
Objective: To explore how to simultaneously detect the content of several phytohormones in cotton root by high performance liquid chromatography(HPLC).Methods: The contents of zeatin(z),ribosylzeatin(ZR),gibberellin(GA3),growth hormone(IAA),abscisin(ABA)were detected by the following conditions: reversed phase column Waters C18(4.6×250mm,5μm) of 35℃,gradient elution by using acetonitrile and triethylamine solution as mobile phase with flow speed 1mL.min-1,the detection wavelength for every substance denpending on it's maximum absorptive summit wavelength during the retension time.Results: Sensitive and recovery rate all were higher,recovery rate of five phytohormones as Z 96.82%,ZR 94.14%,GA3 92.75%,IAA 93.38%,ABA 95.57%.Detection limit of five phytohormones all were lower,Z 0.1μg.mL-1,ZR 0.1μg.mL-1,GA3 0.5μg.mL-1,IAA 0.3 μg.m-1,ABA 0.05μg.mL-1.The content of five phytohormones(Z,ZR,GA3,IAA and ABA)can accuratly be detected.Conclusion: The study established the method which could simultaneously be detected the content of several phytohormones of plant tissue by high performance liquid chromatography(HPLC) through the wavelength change.