The gold nanoparticle size and exposure duration effect on the liver and kidney function of rats: In vivo
The gold nanoparticle size and exposure duration effect on the liver and kidney function of rats: In vivo
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DOI：
10.1016/j.sjbs.2013.01.007
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发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Moussa, Sherif A. Abdelmottaleb
中科院分区：
文献类型：
--
作者：
Abdelhalim, Mohamed Anwar K.;Moussa, Sherif A. Abdelmottaleb
纳米粒子为生物医学的应用提供了极大的可能性，不仅可以提供药物，还可以作为新的诊断和治疗方法。目前，还没有关于体内给药的毒性程度和金纳米颗粒(GNPs)累积程度的数据。本研究旨在探讨GNP的大小和暴露时间对大鼠肝肾功能的影响。方法：健康雄性12周龄Wistar-京都大鼠30只，体重220~240g，取沙特国王大学群体。动物随机分为两个GNP处理组和一个对照组(CG)。将50亩L 10 nm和50 nm GNPs分别经大鼠腹腔注射，连续染毒3d。然后测定谷草转氨酶(AST)、谷氨酰转氨酶(GGT)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、尿素(UREA)、肌酐(CREA)等生化指标。与50 nm GNPs和对照组相比，10 nm GNPs组的AST值显著升高。10nmGNPs和50nmGNPs组大鼠GGT和ALT值与对照组相比明显降低。与10 nm GNPs和对照组相比，50 nm GNPs组GGT和ALT值显著降低。10nmGNPs和50nmGNPs组与对照组相比，ALP值显著降低。与50 nm GNPs相比，10 nm GNPs的ALP值下降幅度更大。在本研究中，与对照组相比，10和50 nm GNPs照射后，尿素和CREA水平升高不明显。结论：暴露3天后，酶AST的升高和ALP的降低与GNPs(10 Nm)的尺寸依赖性较小，而GGT和ALT酶的降低则较大(50 Nm)的GNPs的尺寸依赖性。与对照组相比，10 nm和50 nm GNPs照射3d后，尿素水平和CREA值无明显变化。10 nm和50 nm GNPs短时间照射3d，仅引起肝脏某些酶活性的显著变化，而肾脏则无明显变化。这项研究表明，GNPs的合成和代谢以及肝脏的保护将是未来金基纳米材料医学应用的更重要的问题。(C)2013年，爱思唯尔公司代表沙特国王大学制作和主办。
Nanoparticles (NPs) offer a great possibility for biomedical application, not only to deliver pharmaceutics, but also to be used as novel diagnostic and therapeutic approaches. Currently, there are no data available regarding to what extent the degree of the toxicity and the accumulation of gold nanoparticles (GNPs) are present in in vivo administration. This study aimed to address the GNP size and exposure duration effect on the liver and kidney function of rats: in vivo.Methods: A total of 30 healthy male Wistar-Kyoto rats of the same age (12 weeks old) and weighing 220-240 g of King Saud University colony were used. Animals were randomly divided into groups, two GNP-treated rat groups and one control group (CG). The 50 mu l of 10 and 50 nm GNPs was intraperitoneally administered in rats for exposure duration of 3 days. Then, several biochemical parameters such as aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), alanine transaminase (ALT), alkaline phosphatase (ALP), urea (UREA) and creatinine (CREA) were evaluated.Results: In this study, the AST values increased with the administration of 10 and 50 nm GNPs compared with the control. The AST values significantly increased with 10 nm GNPs compared with 50 nm GNPs and control. The GGT and ALT values decreased with the administration of 10 and 50 nm GNPs compared with the control. The GGT and ALT values significantly decreased with 50 nm GNPs compared with 10 nm GNPs and control. The ALP values significantly decreased with the administration of 10 and 50 nm GNPs compared with the control. The decrease in ALP values with 10 nm GNPs was higher than those compared with 50 nm GNPs. In this study, the levels of UREA and CREA values increased in a non significant manner after the administration of 10 and 50 nm GNPs compared with the control.Conclusions: This study demonstrates that the increase in the enzymes AST and the decrease in ALP are smaller GNPs (10 nm) size-dependent for exposure duration of 3 days; while the decrease in the enzymes GGT and ALT are bigger GNPs (50 nm) size-dependent. The levels of UREA and CREA values indicated no significant changes with the administration of 10 and 50 nm GNPs for exposure duration of 3 days compared with the control. The administration of 10 and 50 nm GNPs for short exposure duration of 3 days induced only significant variations with some liver enzymes while kidney showed no significant variations. This study suggests that synthesis and metabolism of GNPs as well as the protection of the liver will be more important issues for medical applications of gold-based nanomaterials in future. (C) 2013 Production and hosting by Elsevier B.V. on behalf of King Saud University.