Development of a Rat Model of Patulous Eustachian Tube by Mandibular Nerve Resection
Development of a Rat Model of Patulous Eustachian Tube by Mandibular Nerve Resection
复制标题
下颌神经切除术建立大鼠咽鼓管扩张模型
DOI：
10.1016/j.anl.2019.03.001
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发表时间：
2019
影响因子：
1.7
通讯作者：
Oshima T
中科院分区：
文献类型：
--
作者：
Harada H;Hirai R;Matsumoto T;Oshima T
咽鼓管扩张（PET）目前使用各种保守或手术方法治疗。为了进一步阐明PET的病理学并建立新的治疗方法，有必要开发动物模型。本研究的目的是开发一个高度可重复性和可持续的PET大鼠模型，下颌神经切除术。在外部切口后，在翼窝中确定卵圆孔处的下颌神经主干，并切除其分支。手术在右侧进行，未手术的左侧用作对照。为了确定咽鼓管（ET）功能，使用充气法测量术后第16周的被动开放压（POP）。将手术侧和对照侧的POP变化与术前水平进行统计学比较。此外，标本的ET及其周围组织在第16周手术后进行了形态学评价，在8只rates.ResultsOn对照侧，POP没有显着降低，在整个16周手术后相比，术前水平。相反，在手术侧，POP在第2周显著降低，并持续低于术前水平，直至术后至少第16周。在13只大鼠中，手术侧POP下降10%或更多。结论切除大鼠下颌神经是一种制备PET动物模型的有效方法。这表明，这种大鼠模型可能有助于验证PET的新治疗方法。
ObjectivePatulous eustachian tube (PET) is currently treated using a variety of conservative or surgical approaches. To further elucidate the pathology of PET and to establish new therapies, the development of an animal model is necessary. The objective of this study was to develop a highly reproducible and sustainable rat model of PET by mandibular nerve resection.MethodsSixteen rats underwent mandibular nerve resection. Following an external incision, the main trunk of the mandibular nerve at the foramen ovale was identified in the pterygoid fossa, and its branches were resected. This surgery was performed on the right side, with the unoperated left side used as the control. To determine eustachian tube (ET) function, passive opening pressure (POP) was measured using inflation method up to Week 16 post-surgery. Changes in POP were statistically compared to the preoperative level on the operated and control sides. In addition, specimens of the ET and its surrounding tissue at Week 16 post-surgery were prepared for morphological evaluation in eight rats.ResultsOn the control side, POP did not significantly decrease across the 16 weeks post-surgery compared to the preoperative level. In contrast, on the operated side, POP was significantly decreased at Week 2 and continued to be lower than the preoperative level until at least Week 16 post-surgery. POP dropped 10% or more on the operated side examined in thirteen rats. Histologically, the medial pterygoid muscle was atrophied on the operated side mainly.ConclusionMandibular nerve resection in rats may be an effective method for generating an animal model of PET. It was suggested that this rat model may be useful for verifying new treatments for PET.