Nucleolar dynamics and interactions with nucleoplasm in living cells
Nucleolar dynamics and interactions with nucleoplasm in living cells
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DOI：
10.7554/elife.47533
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发表时间：
2019-11-26
期刊：
影响因子：
7.7
通讯作者：
Zidovska, Alexandra
中科院分区：
文献类型：
--
作者：
Caragine, Christina M.;Haley, Shannon C.;Zidovska, Alexandra
液-液相分离（LLPS）已被认为是关键的细胞组织原理之一，并被证明负责无膜细胞器（如核仁）的形成。尽管人们发现核仁的行为类似于液滴，但 LLPS 的许多后果，包括核仁动力学以及与周围液体的相互作用仍有待揭示。在这里，我们研究人类核仁在体内的运动，同时监测核仁-核质界面的形状。我们揭示了两种类型的核仁对动力学：聚结之前的意外相关运动和否则的独立运动。这种令人惊讶的动力学导致核仁体积分布 p(V) 类似于 V-1，目前任何理论都无法解释。此外，我们发现核仁-核质界面由 ATP 依赖性过程维持，并且容易受到染色质转录和包装变化的影响。我们的结果通过显示周围核质对活细胞核仁的影响来扩展和丰富 LLPS 框架。
Liquid-liquid phase separation (LLPS) has been recognized as one of the key cellular organizing principles and was shown to be responsible for formation of membrane-less organelles such as nucleoli. Although nucleoli were found to behave like liquid droplets, many ramifications of LLPS including nucleolar dynamics and interactions with the surrounding liquid remain to be revealed. Here, we study the motion of human nucleoli in vivo, while monitoring the shape of the nucleolus-nucleoplasm interface. We reveal two types of nucleolar pair dynamics: an unexpected correlated motion prior to coalescence and an independent motion otherwise. This surprising kinetics leads to a nucleolar volume distribution, p(V) similar to V-1, unaccounted for by any current theory. Moreover, we find that nucleolus-nucleoplasm interface is maintained by ATP-dependent processes and susceptible to changes in chromatin transcription and packing. Our results extend and enrich the LLPS framework by showing the impact of the surrounding nucleoplasm on nucleoli in living cells.