Efficient and rapid in-vitro plantlet regeneration via somatic embryogenesis in ornamental bananas (Musa spp.)
Efficient and rapid in-vitro plantlet regeneration via somatic embryogenesis in ornamental bananas (Musa spp.)
复制标题
DOI：
10.2478/s11756-019-00358-0
复制
发表时间：
2019-09
期刊：
影响因子：
1.5
通讯作者：
Nandhakumar Natarajan;Sathish Sundararajan;S. Ramalingam;Perinba Suresh Chellakan
中科院分区：
文献类型：
--
作者：
Nandhakumar Natarajan;Sathish Sundararajan;S. Ramalingam;Perinba Suresh Chellakan
建立了一种简单有效的三种观赏香蕉体细胞胚胎发生再生植株的方法。试管苗繁殖，尤其是体细胞胚胎发生已成为获得观赏香蕉均质种植材料的首选方法。在本研究中，通过体细胞胚胎发生获得了三种观赏香蕉的真实型植株。以未成熟的雄性花蕾为外植体，研究了侧叶芭蕾、贝卡利芭蕾和绒花芭蕾的生长发育规律。评价分体细胞胚胎发生、体细胞胚胎诱导和体细胞胚胎成熟两个阶段进行。胚性愈伤组织在25±1℃下干燥2 h，胚胎诱导率和成熟率高于未干燥的胚性愈伤组织。产生的植株经过硬化，并用SRAP标记证实了植株的遗传保真度。观察到，通过体细胞胚胎发生获得的香蕉植株表现出正常的表型，与由单个优良母株生长的植株相同。在本研究中，雄花芽在6至7个月内成功地建立了植株，与其他涉及不同种类香蕉的报道相比，这是一个相对较短的时间。开发的方案可能对这些具有重要商业价值的观赏香蕉的大规模微繁殖研究有用。
A simple and efficient protocol developed for plantlet regeneration through in vitro somatic embryogenesis in three ornamental bananas. In vitro propagation, especially somatic embryogenesis has been a method of choice to obtain homogenous planting material in ornamental bananas. In the present investigation, true-to-type plants via somatic embryogenesis were achieved in three ornamental bananas viz. Musa laterita, Musa beccarii and Musa velutina using immature male flower bud as explants. The evaluation was carried out at two stages of somatic embryogenesis, induction, and maturation of somatic embryos. Embryogenic calli desiccated up to 2 h at 25 ± 1 °C resulted in higher frequencies of embryo induction and maturation compared to non-desiccated embryos. The plantlets generated were hardened, and the genetic fidelity of the plantlets was confirmed using SRAP markers. It was observed that banana plants derived via somatic embryogenesis displayed normal phenotype as that of plants grown from a single elite mother plant. In the present study, the successful establishment of plants from male flower bud was achieved in 6 to 7 months which is a relatively short duration compared to other reports involving different species of banana. The protocol developed could be useful for large-scale micropropagation studies in these commercially important ornamental bananas.