Equine Adipose-Derived Mesenchymal Stromal Cells Release Extracellular Vesicles Enclosing Different Subsets of Small RNAs
Equine Adipose-Derived Mesenchymal Stromal Cells Release Extracellular Vesicles Enclosing Different Subsets of Small RNAs
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DOI：
10.1155/2019/4957806
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发表时间：
2019-01-01
影响因子：
4.3
通讯作者：
Pascucci, Luisa
中科院分区：
文献类型：
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作者：
Capomaccio, Stefano;Cappelli, Katia;Pascucci, Luisa
背景资料。马脂肪间充质基质细胞(e-AdMSC)表现出诱人的再生特性，与细胞外囊泡(EV)的传递密切相关，EV包裹着包括RNA在内的不同分子。在这项研究中，我们研究了e-AdMSC产生的EVS的小RNA含量，目的是推测它们可能的生物学作用。方法：研究方法。取4例受试者的e-AdMSC条件培养液，超速离心法获得EVS。用透射电子显微镜和扫描电子显微镜对其尺寸和纳米结构进行了表征。提取RNA，富含小RNA(
Background. Equine adipose-derived mesenchymal stromal cells (e-AdMSC) exhibit attractive proregenerative properties strongly related to the delivery of extracellular vesicles (EVs) that enclose different kinds of molecules including RNAs. In this study, we investigated small RNA content of EVs produced by e-AdMSC with the aim of speculating on their possible biological role. Methods. EVs were obtained by ultracentrifugation of the conditioned medium of e-AdMSC of 4 subjects. Transmission electron microscopy and scanning electron microscopy were performed to assess their size and nanostructure. RNA was isolated, enriched for small RNAs (