Seasonal expressions of ER alpha, ER beta, EGF, EGFR, PI3K and Akt in the scent glands of the muskrats (Ondatra zibethicus)
Seasonal expressions of ER alpha, ER beta, EGF, EGFR, PI3K and Akt in the scent glands of the muskrats (Ondatra zibethicus)
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麝香鼠 (Ondatra zibethicus) 气味腺中 ER α、ER β、EGF、EGFR、PI3K 和 Akt 的季节性表达
DOI：
10.1016/j.jsbmb.2021.105961
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发表时间：
2021
影响因子：
4.1
通讯作者：
Weng Qiang
中科院分区：
文献类型：
--
作者：
Xie Wenqian;Zhao Xinyu;Guo Li;Han Yingying;Yuan Zhengrong;Zhang Haolin;Weng Qiang
表皮生长因子（Epidermal growth factor， EGF）是一种重要的自分泌和/或旁分泌类固醇激素的介质，用于刺激哺乳动物的生长和分化。本研究旨在研究雌性激素受体α (ERα)、雌性激素受体β (ERβ)、EGF、表皮生长因子受体（EGFR）、磷脂酰肌醇3-激酶（PI3K）和蛋白激酶B （Akt）在繁殖季节和非繁殖季节在麝鼠气味腺中的季节性表达。在组织学上，两个季节的气味腺中都有腺细胞、间质细胞和上皮细胞三种类型的细胞。免疫组化结果显示，在繁殖期和非繁殖期，不同类型的嗅觉腺细胞中均存在ERα、ERβ、EGF、EGFR、PI3K和Akt。麝鼠在繁殖期和非繁殖期嗅觉腺组织的转录组数据显示，不同的季节变化可能与雌激素- egfr信号通路有关。ERα、ERβ、EGF、EGFR、PI3K基因表达量在繁殖期较非繁殖期升高，Akt基因表达量较非繁殖期降低。血清和嗅觉腺组织中17β-雌二醇（E2）浓度在繁殖季节显著高于非繁殖季节。综上所述，我们的研究结果表明，EGFR信号通路可能与er信号通路协同调节嗅觉腺功能的季节性变化。
Epidermal growth factor (EGF) is an important autocrine and/or paracrine mediator of steroid hormones to stimulate growth and differentiation in mammals. The aim of this study is to investigate seasonal expressions of estrogen receptor α (ERα), estrogen receptor β (ERβ), EGF, epidermal growth factor receptor (EGFR), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt) in the scent glands of the muskrats during the breeding and non-breeding seasons. Histologically, three types of cells including the glandular cells, interstitial cells and epithelial cells were identified in the scent glands in both seasons. Immunohistochemical results showed that ERα, ERβ, EGF, EGFR, PI3K and Akt were presented in the different types of cells of the scent glands during the breeding and non-breeding seasons. Transcriptome data of the scent glandular tissues from muskrats in the breeding and non-breeding seasons showed that differential seasonal changes might be related to the estrogen-EGFR signaling pathway. The gene expression levels of ERα, ERβ, EGF, EGFR, PI3K were increased, while the gene expression level of Akt were decreased in the breeding season than those in the non-breeding season. Besides, the concentrations of 17β-estradiol (E2) in the serum and the scent glandular tissues were remarkably higher in the breeding season than those of the non-breeding season. Taken together, our results suggested that EGFR signaling pathway may coordinate with ERs signaling to regulate the seasonal changes of the scent glandular functions.