Effects of Tolypocladium cylindrosporum and its secondary metabolites, efrapeptins, on the immune system of Galleria mellonella larvae
Effects of Tolypocladium cylindrosporum and its secondary metabolites, efrapeptins, on the immune system of Galleria mellonella larvae
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DOI：
10.1080/09583150400015961
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发表时间：
2005-02-01
影响因子：
1.4
通讯作者：
Bandani, AR
中科院分区：
文献类型：
--
作者：
Bandani, AR
研究了圆柱孢霉及其代谢物EraPeptins在体内外与昆虫免疫防御的相互作用。在不同的吞噬研究中，使用了标记了异硫氰酸荧光素(FITC)的蜡状芽胞杆菌。体外研究表明，卵肽对斑潜蝇血细胞的吞噬活性有明显的抑制作用。这种反应与剂量有关。EfraPeptins显著减少了注射酵母多糖上清液后形成的结节数量。血细胞裂解物(HLS)中的酚氧化酶(PO)激活系统不受efraPeptins的影响。体内研究也表明，幼虫注射EFRAPTINS后1h和6h对PO活性和血细胞总数(THC)没有影响。但注射后12h，HLS的PO活性受到明显抑制。幼虫注射柱状弯孢霉孢子至注射后24 h，其PO活性和THC均未见明显下降。带孢子的幼虫处理48h后，PO活性受到抑制，THC降低。这项研究表明，efraPeptins可能会干扰可能发生在特定血细胞质膜上的配体-受体相互作用。
Interactions between Tolypocladium cylindrosporum (Deuteromycetes), its metabolite, efrapeptins, and the insect immune defense were investigated in vivo and in vitro. In the different phagocytosis studies, Bacillus cereus spores which had been labelled with fluorescein-isothiocyanate (FITC) were used. In vitro studies showed that efrapeptins inhibit phagocytic activity of Galleria mellonella (Lepidoptera: Pyralidae) haemocytes. The response was dose-related. Efrapeptins significantly reduced the number of nodules formed in response to an injection of zymosan supernatant. Phenoloxidase (PO) activation system contained in haemocyte lysate (HLS) was not affected by efrapeptins. In vivo studies when larvae were injected with efrapeptins also revealed that efrapeptins did not affect PO activities and total haemocyte count (THC) after 1 and 6 h post-injection. However, 12 h post-injection there was a significant inhibition of PO activities in HLS. There was also no significant reduction of PO activities and THC when larvae were injected with Tolypocladium cylindrosporum spores until 24 h post-injection. However, PO activities were suppressed and THC reduced 48 h post-treatment of larvae with spores. This study suggests that efrapeptins may interfere with the ligand-receptor interactions that are likely to occur at the plasma membrane of specific haemocytes.