Xanthones induce cell-cycle arrest and apoptosis in human colon cancer DLD-1 cells
Xanthones induce cell-cycle arrest and apoptosis in human colon cancer DLD-1 cells
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DOI：
10.1016/j.bmc.2005.06.065
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发表时间：
2005-11-01
影响因子：
3.5
通讯作者：
Nozawa, Y
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Akao, Y;Nozawa, Y
我们研究了四种结构相似的烯丙基化山酮，α -山竹苷、β -山竹苷、γ -山竹苷和甲氧基-p-山竹苷在人结肠癌DLD-1细胞中的抗增殖作用。这些山酮的羟基和甲氧基数量不同。20 μ M时，除甲氧基-山竹苷外，其余3种山酮均能明显抑制细胞生长，其抗肿瘤效果与羟基数量呈正相关。Hoechst 33342核染色和核小体dna凝胶电泳显示α -和γ -山竹苷的抗增殖作用与细胞凋亡有关，而β -山竹苷的抗增殖作用与细胞凋亡无关。研究还表明，它们的抗增殖作用通过影响细胞周期蛋白、cdc2和p27的表达而与细胞周期阻滞有关；α -山竹苷和β -山竹苷阻滞G1， γ -山竹苷阻滞S。这些发现为开发山酮类药物作为防癌药物及与抗癌药物联合治疗提供了相关依据。(c) 2005 Elsevier Ltd版权所有。
We investigated the antiproliferative effects of four structurally similar prenylated xanthones, alpha-mangostin, beta-mangostin, gamma-mangostin, and methoxy-p-mangostin, in human colon cancer DLD-1 cells. These xanthones differ in the number of hydroxyl and methoxy groups. Except for methoxy-beta-mangostin, the other three xanthones strongly inhibited cell growth at 20 mu M and their antitumor efficacy was correlated with the number of hydroxyl groups. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that the antiproliferative effects of alpha- and gamma-mangostin, but not that of beta-mangostin, were associated with apoptosis. It was also shown that their antiproliferative effects were associated with cell-cycle arrest by affecting the expression of cyclins, cdc2, and p27; G1 arrest was by alpha-mangostin and beta-mangostin, and S arrest by gamma-mangostin. These findings provide a relevant basis for the development of xanthones as an agent for cancer prevention and combination therapy with anti-cancer drugs. (c) 2005 Elsevier Ltd. All rights reserved.