Chronic coffee consumption in the diet-induced obese rat: impact on gut microbiota and serum metabolomics
Chronic coffee consumption in the diet-induced obese rat: impact on gut microbiota and serum metabolomics
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DOI：
10.1016/j.jnutbio.2013.12.009
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发表时间：
2014-04-01
影响因子：
5.6
通讯作者：
Shearer, Jane
中科院分区：
文献类型：
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作者：
Cowan, Theresa E.;Palmnaes, Marie S. A.;Shearer, Jane
流行病学数据证实，经常饮用咖啡与 2 型糖尿病患病率之间存在很强的负相关关系。咖啡最初在胃和小肠中吸收，但在结肠中通过肠道微生物群进一步发酵。咖啡多酚的生物利用度、产量和生物活性部分受到肠道微生物群的调节。本研究的目的是确定长期饮用咖啡是否可以减轻高脂肪饮食引起的负面肠道微生物群和代谢组学变化。雄性 Sprague-Dawley 大鼠被随机分为粗粮（12% kcal 脂肪）或高脂（60% kcal 脂肪）饮食。每组进一步分为水或含咖啡因的咖啡，为期 10 周。高脂肪喂养的大鼠喝咖啡与体重、肥胖、肝脏甘油三酯和能量摄入的降低有关。尽管身体成分更有利，但大鼠表现出严重的全身胰岛素抵抗，这可能是由于咖啡因。咖啡摄入减弱了通常与高脂肪喂养相关的厚壁菌门 (F) 与拟杆菌门 (B) 比率和梭状芽胞杆菌 XI 的增加，但也导致肠杆菌水平增加。在血清代谢组中，咖啡具有明显的影响，增加芳香族和循环短链脂肪酸的水平，同时降低支链氨基酸的水平。总之，摄入咖啡能够改变高脂肪喂养大鼠的肠道微生物群，尽管鉴于本研究中观察到咖啡会增加胰岛素抵抗，这些变化在降低糖尿病风险方面的作用尚不清楚。 (C) 2014 Elsevier Inc. 保留所有权利。
Epidemiological data confirms a strong negative association between regular coffee consumption and the prevalence of type 2 diabetes. Coffee is initially absorbed in the stomach and small intestine but is further fermented in the colon by gut microbiota. The bioavailability, production and biological activity of coffee polyphenols is modulated, in part, by gut microbiota. The purpose of this study was to determine if chronic coffee consumption could mitigate negative gut microbiota and metabolomic profile changes induced by a high-fat diet. Male Sprague-Dawley rats were randomized to chow (12% kcal fat) or high-fat (60% kcal fat) diet. Each group was further divided into water or caffeinated coffee for 10 weeks. Coffee consumption in high-fat-fed rats was associated with decreased body weight, adiposity, liver triglycerides and energy intake. Despite a more favorable body composition, rats displayed profound systemic insulin resistance, likely due to caffeine. Coffee consumption attenuated the increase in Firmicutes (F)-to-Bacteroidetes (B) ratio and Clostridium Cluster XI normally associated with high-fat feeding but also resulted in augmented levels of Enterobacteria. In the serum metabolome, coffee had a distinct impact, increasing levels of aromatic and circulating short-chain fatty acids while lowering levels of branched-chain amino acids. In summary, coffee consumption is able to alter gut microbiota in high-fat-fed rats although the role of these changes in reducing diabetes risk is unclear given the increased insulin resistance observed with coffee in this study. (C) 2014 Elsevier Inc. All rights reserved.