JNK signalling is necessary for a Wnt- and stem cell-dependent regeneration programme
JNK signalling is necessary for a Wnt- and stem cell-dependent regeneration programme
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DOI：
10.1242/dev.115139
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发表时间：
2015-07-15
期刊：
影响因子：
4.6
通讯作者：
Aboobaker, A. Aziz
中科院分区：
文献类型：
--
作者：
Tejada-Romero, Belen;Carter, Jean-Michel;Aboobaker, A. Aziz
再生涉及在成年生活史的背景下新旧组织的整合。很明显，协调发展的核心保守的信号通路也在再生中发挥核心作用，需要进一步研究保守的信号通路。在这里，我们已经研究了保守的JNK信号级联的作用，在涡虫再生。通过RNAi或药理学抑制消除JNK信号传导阻断后部再生，并且动物不能表达后部标记物。虽然早期损伤诱导的极性标志物的表达不受影响，但后部Wnt表达的后期干细胞依赖性阶段尚未建立。这种缺陷可以通过Hh或Wnt信号通路的过度激活来挽救，以促进后部Wnt活性。总之，我们的数据表明，JNK信号传导需要建立干细胞依赖性Wnt表达后损伤。鉴于六月是已知需要在脊椎动物的Wnt和Wnt靶基因的表达，我们建议，这种相互作用可能是保守的，是一个有益的一部分，涡虫后再生。
Regeneration involves the integration of new and old tissues in the context of an adult life history. It is clear that the core conserved signalling pathways that orchestrate development also play central roles in regeneration, and further study of conserved signalling pathways is required. Here we have studied the role of the conserved JNK signalling cascade during planarian regeneration. Abrogation of JNK signalling by RNAi or pharmacological inhibition blocks posterior regeneration and animals fail to express posterior markers. While the early injury-induced expression of polarity markers is unaffected, the later stem cell-dependent phase of posterior Wnt expression is not established. This defect can be rescued by overactivation of the Hh or Wnt signalling pathway to promote posterior Wnt activity. Together, our data suggest that JNK signalling is required to establish stem cell-dependent Wnt expression after posterior injury. Given that Jun is known to be required in vertebrates for the expression of Wnt and Wnt target genes, we propose that this interaction may be conserved and is an instructive part of planarian posterior regeneration.