THE TS41 MUTATION IN CHINESE-HAMSTER CELLS LEADS TO SUCCESSIVE S PHASES IN THE ABSENCE OF INTERVENING G2, M, AND G1
THE TS41 MUTATION IN CHINESE-HAMSTER CELLS LEADS TO SUCCESSIVE S PHASES IN THE ABSENCE OF INTERVENING G2, M, AND G1
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DOI：
10.1016/0092-8674(92)90594-3
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发表时间：
1992-11-13
期刊：
影响因子：
64.5
通讯作者：
WEINTRAUB, H
中科院分区：
文献类型：
--
作者：
HANDELI, S;WEINTRAUB, H
中国仓鼠细胞的ts41突变首先由Hirschberg和Marcus（1982）分离和表征，他们表明在非允许温度下，细胞积累高达16 C当量的DNA。在这里，我们表明该突变是隐性的，并且在非允许的温度下，细胞正常复制其基因组，但它们不是进入G2、M和G1，而是直接进入第二个S期。进入第二个S期不需要血清，也不受G2检查点或有丝分裂抑制剂的抑制。温度变化实验表明，ts41基因产物在细胞周期中参与两种功能：进入有丝分裂和抑制进入S期。ts41突变似乎定义了一类耦合DNA复制和有丝分裂的顺序事件的细胞周期突变体。
The ts41 mutation of Chinese hamster cells was first isolated and characterized by Hirschberg and Marcus (1982) who showed that at nonpermissive temperature, cells accumulate up to 16C equivalents of DNA. Here we show that the mutation is recessive and at nonpermissive temperature, cells replicate their genome normally, but instead of going on into G2, M, and G1, they pass directly into a second S phase. Entry into a second S phase does not require serum nor is it inhibited by G2 checkpoints or mitotic inhibitors. Temperature-shift experiments suggest that the ts41 gene product participates in two functions in the cell cycle: entry into mitosis and inhibition of entry into S phase. The ts41 mutation seems to define a class of cell cycle mutant that couples the sequential events of DNA replication and mitosis.