Participation of the microsomal electron transport system in mutagenic activation of 4-dimethylaminoazobenzene, 4-methylaminoazobenzene and their 3'-methyl-derivatives.
Participation of the microsomal electron transport system in mutagenic activation of 4-dimethylaminoazobenzene, 4-methylaminoazobenzene and their 3'-methyl-derivatives.
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微粒体电子传递系统参与4-二甲基氨基偶氮苯、4-甲基氨基偶氮苯及其3-甲基衍生物的诱变活化。
DOI：
10.1016/0006-291x(82)92072-1
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发表时间：
1982
影响因子：
3.1
通讯作者：
Y. Tagashira
中科院分区：
文献类型：
--
作者：
S. Igarashi;H. Yonekawa;K. Kawajiri;J. Watanabe;T. Kimura;M. Kodama;C. Nagata;Y. Tagashira
用抗NADPH-细胞色素P-450还原酶、3-甲基胆蒽诱导的主要P-450(MC-P-448)和苯巴比妥诱导的主要P-450(PB-P-450)的单特异性抗体，观察微粒体电子传递系统在4-二甲氨基偶氮苯(DAB)、4-甲氨基偶氮苯(MAB)及其3‘-甲基-甲基-偶氮苯(3’-甲基-DAB和3‘-甲基-MAB)致突变激活中的作用。在Ames试验系统中，抗NADPH-细胞色素P-450还原酶抗体对DAB、MAB、3‘-甲基-DAB和3’-甲基-MAB的致突变性分别抑制94、94、90和87%。MC-P-448抗体对其致突变性的抑制率在90%以上，而PB-P-450的抗体对致突变性的抑制率不到20%。这些结果表明，微粒体电子传递系统，特别是MC-P-448，参与了这些染料的活化。
For the examination of the participation of the microsomal electron transport system in mutagenic activation by 4-dimethylaminoazobenzene (DAB), 4-methylaminoazobenzene (MAB) and their 3′-methyl-derivatives (3′-methyl-DAB and 3′-methyl-MAB), monospecific antibodies to NADPH-cytochrome P-450 reductase, 3-methylcholanthrene-inducible major P-450 (MC-P-448) and phenobarbital-inducible major P-450 (PB-P-450) were used. In Ames' assay system, the antibody to NADPH-cytochrome P-450 reductase inhibited the mutagenicities of DAB, MAB, 3′-methyl-DAB and 3′-methyl-MAB by 94, 94, 90 and 87%, respectively. The antibody to MC-P-448 inhibited their mutagenicities by more than 90%, while the antibody to PB-P-450 inhibited the mutagenicities less than 20%. These results indicate that the microsomal electron transport system, especially MC-P-448, is involved in activation of these dyes.