Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-spanning polymethylene cross-linkage between two head groups of ditetradecylphosphatidylcholine membrane
Membrane properties of amacrocyclic tetraether bisphosphatidylcholine lipid: Effect of a single membrane-spanning polymethylene cross-linkage between two head groups of ditetradecylphosphatidylcholine membrane
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大环四醚双磷脂酰胆碱脂质的膜特性：双十四烷基磷脂酰胆碱膜两个头基之间单跨膜聚亚甲基交联的影响
DOI：
10.1016/j.bbamem.2021.183569
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sonoyama Masashi
中科院分区：
文献类型：
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作者：
Tsuchida Naoyuki;Takagi Toshiyuki;Takahashi Hiroshi;Yoshihara Toshitada;Tobita Seiji;Sonoyama Masashi
古细菌的质膜中含有大量的大环四醚类脂质，这些脂质含有一条或两条长跨膜烃链，在两端连接两个甘油骨架。本研究合成了一种具有单跨膜八碳甲基链的新型无环双磷脂酰胆碱脂，1,1′- o-八碳亚甲基-2,2′-二-o -十四烷基-二-（cn -甘油）-3,3′-二磷酸胆碱（AC-(di-O-C14PC)2），通过与相应的二醚磷脂酰胆碱1,2-二-o -十四烷基- cn -甘油-3-磷酸胆碱（DTPC）形成双层膜进行比较，阐述层间交联对膜性能的影响。几种物理化学技术表明，虽然在凝胶相中采用特别高有序结构的AC-(di-O-C14PC)2单层与DTPC双层相比具有显着高的热致转变温度，但两种磷脂在转变温度以上的流动性是相当的。然而，荧光染料在液相中从AC-(di-O-C14PC)2囊泡内部泄漏被高度抑制。从单长跨膜疏水键和脂质分子的扩散运动两方面讨论了AC-(di-O-C14PC)2单分子膜特性的起源。
The plasma membranes of archaea are abundant in macrocyclic tetraether lipids that contain a single or double long transmembrane hydrocarbon chains connecting the two glycerol backbones at both ends. In this study, a novel amacrocyclic bisphosphatidylcholine lipid bearing a single membrane-spanning octacosamethylene chain, 1,1’-O-octacosamethylene-2,2′-di-O-tetradecyl-bis-(sn-glycero)-3,3′-diphosphocholine (AC-(di-O-C14PC)2), was synthesized to elucidate effects of the interlayer cross-linkage on membrane properties based on comparison with its corresponding diether phosphatidylcholine, 1,2-di-O-tetradecyl-sn-glycero-3-phosphocholine (DTPC), that forms bilayer membrane. Several physicochemical techniques demonstrated that while AC-(di-O-C14PC)2monolayer, which adopts a particularly high-ordered structure in the gel phase, shows remarkably high thermotropic transition temperature compared to DTPC bilayer, the fluidity of both phospholipids above the transition temperature is comparable. Nonetheless, the fluorescent dye leakage from inside the AC-(di-O-C14PC)2vesicles in the fluid phase is highly suppressed. The origin of the membrane properties characteristic of AC-(di-O-C14PC)2monolayer is discussed in terms of the single long transmembrane hydrophobic linkage and the diffusional motion of the lipid molecules.