Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation
Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation
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DOI：
10.1186/s12263-019-0644-8
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发表时间：
2019-06-18
影响因子：
3.5
通讯作者：
Holloway, John W.
中科院分区：
文献类型：
--
作者：
Losol, Purevsuren;Rezwan, Faisal I.;Holloway, John W.
背景证据表明，产前接触 n-3 长链多不饱和脂肪酸 (LCPUFA) 可降低儿童过敏性疾病的发生率。 LCPUFA 是由 FADS1/2 和 ELOVL5 基因编码的去饱和酶和延伸酶催化的膳食前体产生的。 DNA 甲基化调节基因活性，补充脂肪酸可以改变这些基因的 DNA 甲基化 (DNA-M)。我们研究了 DNA-M 以及 FADS1/2 和 ELOVL5 基因的表达是否与儿童过敏和妊娠鱼摄入量相关。我们研究了来自英国怀特岛第三代队列的 170 名参与者。通过问卷调查评估表型数据和暴露情况。使用 Illumina Infinium HumanMmethylation450 和 EPIC Beadchips 对脐带血样本中的全基因组 DNA-M 进行定量。 FADS 基因簇中的 5 个 SNP（单核苷酸多态性）和 ELOVL5 中的 1 个 SNP 在后代中进行了基因分型。测定子代脐血中FADS基因表达量。结果妊娠期鱼类摄入量与cg12517394甲基化水平升高显着相关（P=0.049），与FADS1 mRNA水平呈正相关（P=0.021）。 ELOVL5 rs2397142 与湿疹 (P=0.011) 以及 cg11748354 和 cg24524396 的甲基化显着相关 (P
BackgroundEvidence suggests that prenatal exposure to n-3 long-chain polyunsaturated fatty acids (LCPUFA) reduces the incidence of allergic disease in children. LCPUFAs are produced from dietary precursors catalyzed by desaturases and elongases encoded by the FADS1/2 and ELOVL5 genes. DNA methylation regulates gene activity and fatty acid supplementation could alter DNA methylation (DNA-M) at these genes. We investigated whether DNA-M and expression of the FADS1/2 and ELOVL5 genes were associated with allergy in children and gestational fish intake. We studied 170 participants from the Isle of Wight 3rd Generation Cohort, UK. Phenotype data and exposure was assessed by questionnaires. Genome-wide DNA-M in cord blood samples was quantified using the Illumina Infinium HumanMethylation450 and EPIC Beadchips. Five SNPs (single-nucleotide polymorphisms) in the FADS gene cluster and one SNP in ELOVL5 were genotyped in offspring. FADS gene expression in offspring cord blood was determined.ResultsGestational fish intake was significantly associated with increased methylation of cg12517394 (P=0.049), which positively correlated with FADS1 mRNA levels (P=0.021). ELOVL5 rs2397142 was significantly associated with eczema (P=0.011) and methylation at cg11748354 and cg24524396 (P