CAFFEINE-INDUCED UNCOUPLING OF MITOSIS FROM THE COMPLETION OF DNA-REPLICATION IN MAMMALIAN-CELLS
CAFFEINE-INDUCED UNCOUPLING OF MITOSIS FROM THE COMPLETION OF DNA-REPLICATION IN MAMMALIAN-CELLS
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DOI：
10.1126/science.2422760
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发表时间：
1986-06-06
期刊：
影响因子：
56.9
通讯作者：
PARDEE, AB
中科院分区：
文献类型：
--
作者：
SCHLEGEL, R;PARDEE, AB
研究表明，在 DNA 复制（S 期）完成之前，咖啡因会诱导哺乳动物细胞发生有丝分裂事件。当接触咖啡因时，在早期 S 期停滞的同步 BHK 细胞会经历过早的染色体浓缩、核膜破裂、形态四舍五入和有丝分裂特异性磷蛋白合成。这些有丝分裂反应仅在细胞进入 S 期且 DNA 合成被抑制超过 70% 时发生。 蛋白质合成抑制剂可以阻止这些咖啡因诱导的事件，而 RNA 合成抑制剂则几乎没有作用。这些结果表明，当 DNA 复制受到抑制时，咖啡因会诱导有丝分裂相关 RNA 的翻译或稳定其在 S 期细胞中积累的蛋白质产物。化学操纵有丝分裂开始的能力对于研究哺乳动物细胞中该事件的调节应该是有用的。
Caffeine was shown to induce mitotic events in mammalian cells before DNA replication (S phase) was completed. Synchronized BHK cells that were arrested in early S phase underwent premature chromosome condensation, nuclear envelope breakdown, morphological rounding up, and mitosis-specific phosphoprotein synthesis when they were exposed to caffeine. These mitotic responses occurred only after the cells had entered S phase and only while DNA synthesis was inhibited by more than 70 percent. Inhibitors of protein synthesis blocked these caffeine-induced events, while inhibitors of RNA synthesis had little effect. These results suggest that caffeine induces the translation or stabilizes the protein product (or products) of mitosis-related RNA that accumulates in S-phase cells when DNA replication is suppressed. The ability to chemically manipulate the onset of mitosis should be useful for studying the regulation of this event in mammalian cells.