Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos.
Isolation and expression of the human gametocyte-specific factor 1 gene (GTSF1) in fetal ovary, oocytes, and preimplantation embryos.
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DOI：
10.1007/s10815-016-0795-0
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发表时间：
2017-01
影响因子：
3.1
通讯作者：
Picton HM
中科院分区：
文献类型：
--
作者：
Huntriss J;Lu J;Hemmings K;Bayne R;Anderson R;Rutherford A;Balen A;Elder K;Picton HM
在其他物种中，配子细胞特异性因子1已被证明是通过Piwi相互作用RNA（皮尔纳）途径沉默逆转录转座子所必需的。在这项研究中，我们的目的是分离和评估的配子细胞特异性因子1（GTSF 1）基因在人类女性生殖系和植入前胚胎的转录本的表达。从人胎儿卵巢和睾丸、人卵母细胞和植入前胚胎以及从成人卵巢皮质活检分离的卵泡中获得的互补DNA（cDNA）文库被用作PCR、克隆和测序的模板，以及GTSF 1表达的真实的实时PCR实验。 从人卵母细胞和植入前胚胎中分离出覆盖整个编码区的GTSF 1 cDNA克隆。GTSF 1 mRNA表达检测存档的cDNA从分期的人卵巢卵泡，生发泡（GV）阶段的卵母细胞，中期II卵母细胞，桑椹胚和囊胚阶段的植入前胚胎。在成年雌性种系中，GV卵母细胞中的表达最高。还评估了人胎儿卵巢中的GTSF 1 mRNA表达，并观察到在妊娠期间从8周至21周增加，在此期间卵原细胞进入减数分裂并首次发生原始卵泡形成。在人胎儿睾丸中，GTSF 1的表达也从8到19周增加。据我们所知，这份报告是第一个描述人类GTSF 1基因在人类配子和植入前胚胎中的表达。本文的在线版本（doi：10.1007/s10815-016-0795-0）包含补充材料，可供授权用户使用。
Gametocyte-specific factor 1 has been shown in other species to be required for the silencing of retrotransposons via the Piwi-interacting RNA (piRNA) pathway. In this study, we aimed to isolate and assess expression of transcripts of the gametocyte-specific factor 1 (GTSF1) gene in the human female germline and in preimplantation embryos. Complementary DNA (cDNA) libraries from human fetal ovaries and testes, human oocytes and preimplantation embryos and ovarian follicles isolated from an adult ovarian cortex biopsy were used to as templates for PCR, cloning and sequencing, and real time PCR experiments of GTSF1 expression. GTSF1 cDNA clones that covered the entire coding region were isolated from human oocytes and preimplantation embryos. GTSF1 mRNA expression was detected in archived cDNAs from staged human ovarian follicles, germinal vesicle (GV) stage oocytes, metaphase II oocytes, and morula and blastocyst stage preimplantation embryos. Within the adult female germline, expression was highest in GV oocytes. GTSF1 mRNA expression was also assessed in human fetal ovary and was observed to increase during gestation, from 8 to 21 weeks, during which time oogonia enter meiosis and primordial follicle formation first occurs. In human fetal testis, GTSF1 expression also increased from 8 to 19 weeks. To our knowledge, this report is the first to describe the expression of the human GTSF1 gene in human gametes and preimplantation embryos. The online version of this article (doi:10.1007/s10815-016-0795-0) contains supplementary material, which is available to authorized users.