Expression of an intracisternal A-particle-like element in rat ovary.
Expression of an intracisternal A-particle-like element in rat ovary.
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大鼠卵巢中脑池内 A 颗粒样元件的表达。
DOI：
10.1006/bbrc.2000.3759
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Park-Sarge,OK
中科院分区：
文献类型：
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作者：
Graham,KM;Ko,C;Park,KS;Sarge,K;Park-Sarge,OK
我们从卵巢颗粒细胞中分离出大鼠脑池内 A 颗粒元件 (IAP) 样元件 (IAP-LE)，该元件似乎与大鼠 EST 克隆 AA964260 相同。编译的 cDNA 序列包含几个推定的框内翻译起始密码子，其中最大的能够编码 365 个氨基酸的蛋白质，在 N 末端有一个逆转录酶结构域，在中间有一个二分核定位信号序列。 Northern 印迹显示主要~7 Kb 转录本和次要~5 Kb 转录本在卵巢中大量表达。使用促性腺激素处理的未成熟大鼠和定期循环的成年大鼠的卵巢进行的原位杂交组织化学显示，该转录物主要定位于所有健康卵泡（包括初级卵泡）和新形成的健康黄体的颗粒细胞。 IAP-LE 基因的这种细胞特异性表达模式与几种已知的逆转录病毒元件的表达模式不同，表明 IAP-LE 基因潜在的新功能重要性。综上所述，我们的结果证明了新型 IAP-LE 在大鼠颗粒细胞中的丰富且细胞特异性表达。
We have isolated a rat intracisternal-A particle element (IAP)-like element (IAP-LE) from ovarian granulosa cells that appears to be identical to the rat EST clone AA964260. The compiled cDNA sequences contain several putative in-frame translation initiation codons with the largest capable of encoding a 365 amino acid protein with a reverse transcriptase domain in the N-terminus as well as a bipartite nuclear localization signal sequence in the middle. Northern blotting shows a major ∼7 Kb transcript and a minor ∼5 Kb transcript that are abundantly expressed in the ovary. In situ hybridization histochemistry using ovaries from gonadotropin-treated immature rats and regularly cycling adult rats show that this transcript is predominantly localized to granulosa cells of all healthy follicles, including primary follicles, and to newly-formed and healthy corpora lutea. This cell-specific expression pattern of the IAP-LE gene is distinct from those of the several known retroviral elements, suggesting the potentially novel functional importance of the IAP-LE gene. Taken together, our results demonstrate abundant and cell-specific expression of a novel IAP-LE in rat granulosa cells.