Crystal structure of Sec10, a subunit of the exocyst complex.
Crystal structure of Sec10, a subunit of the exocyst complex.
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DOI：
10.1038/srep40909
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发表时间：
2017-01-18
影响因子：
4.6
通讯作者：
Fukai S
中科院分区：
文献类型：
--
作者：
Chen J;Yamagata A;Kubota K;Sato Y;Goto-Ito S;Fukai S
外囊复合物是由Sec3、Sec5、Sec6、Sec8、Sec10、Sec15、Exo70和Exo84组成的异八聚体蛋白质复合物。该复合物通过与磷脂酰肌醇 4,5-二磷酸和小 GTP 酶相互作用，在将分泌囊泡运输至质膜的过程中发挥重要作用。迄今为止，每个亚基的近全长结构信息仅限于 Exo70，尽管 Sec6、Sec15 和 Exo84 的 C 端半结构以及 Sec3、Sec5 和 Exo84 的小 GTPase 结合域的结构已被报道。在这里，我们以 2.73 Å 的分辨率报告了近全长斑马鱼 Sec10 (zSec10) 的晶体结构。 zSec10 的结构由串联反平行螺旋束组成，形成直杆，就像其他外囊亚基的螺旋核心区域一样。该结构提供了 Sec10 的第一个原子细节，这可能有助于该亚基和外囊复合体未来的功能和结构研究。
The exocyst complex is a heterooctameric protein complex composed of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84. This complex plays an essential role in trafficking secretory vesicles to the plasma membrane through its interaction with phosphatidylinositol 4,5-bisphosphate and small GTPases. To date, the near-full-length structural information of each subunit has been limited to Exo70, although the C-terminal half structures of Sec6, Sec15 and Exo84 and the structures of the small GTPase-binding domains of Sec3, Sec5 and Exo84 have been reported. Here, we report the crystal structure of the near-full-length zebrafish Sec10 (zSec10) at 2.73 Å resolution. The structure of zSec10 consists of tandem antiparallel helix bundles that form a straight rod, like helical core regions of other exocyst subunits. This structure provides the first atomic details of Sec10, which may be useful for future functional and structural studies of this subunit and the exocyst complex.