Selective and Context-Dependent Social and Behavioral Effects of Δ9-Tetrahydrocannabinol in Weakly Electric Fish.
Selective and Context-Dependent Social and Behavioral Effects of Δ9-Tetrahydrocannabinol in Weakly Electric Fish.
复制标题
α9-四氢大麻酚对弱电鱼的选择性和上下文相关的社会和行为影响。
DOI：
10.1159/000490171
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Marsat,Gary
中科院分区：
文献类型：
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作者：
Neeley,Brandon;Overholt,Tyler;Artz,Emily;Kinsey,StevenG;Marsat,Gary
大麻素（CB）受体广泛存在于神经系统中，并影响各种行为。在研究行为的神经基础方面，弱电鱼一直是一个有用的模型系统，但我们对CB系统所起的作用一无所知。在这里，我们确定了一种非选择性CB受体激动剂，即Δ 9-四氢大麻酚（THC），在弱电鱼类Apte-ronotus leptorhynchus的整体行为效应。使用涉及社会刺激的各种行为范例，我们表明，THC减少运动行为，在许多物种中，并影响沟通和社会行为。在不同的实验中，我们发现，发出通信信号（啁啾）和寻求社会互动的倾向受到上下文相关方式的影响。我们明确地测试了这一假设，通过比较THC注射在鱼中的行为影响，放置在一个新的与熟悉的社会和物理环境。在熟悉的情况下，注射THC的鱼比对照鱼不太可能啁啾，但在新的情况下则不然。只有在新的环境中，与其他鱼类密切接近的趋势才受到影响，对照鱼比THC注射的鱼更容易聚集。通过识别CB激动剂影响的行为，我们的研究可以指导进一步的比较和神经生理学研究的CB系统的作用，使用弱电鱼作为模型。
Cannabinoid (CB) receptors are widespread in the nervous system and influence a variety of behaviors. Weakly electric fish have been a useful model system in the study of the neural basis of behavior, but we know nothing of the role played by the CB system. Here, we determine the overall behavioral effect of a nonselective CB receptor agonist, namely Δ 9-tetrahydrocannabinol (THC), in the weakly electric fish Apte-ronotus leptorhynchus. Using various behavioral paradigms involving social stimuli, we show that THC decreases locomotor behavior, as in many species, and influences communication and social behavior. Across the different experiments, we found that the propensity to emit communication signals (chirps) and seek social interactions was affected in a context-dependent manner. We explicitly tested this hypothesis by comparing the behavioral effects of THC injection in fish placed in a novel versus a familiar social and physical environment. THC-injected fish were less likely to chirp than control fish in familiar situations but not in novel ones. The tendency to be in close proximity to other fish was affected only in novel environments, with control fish clustering more than THC-injected ones. By identifying behaviors affected by CB agonists, our study can guide further comparative and neurophysiological studies of the role of the CB system using a weakly electric fish as a model.