Vitality of pancreatic islets labeled for magnetic resonance imaging with iron particles
Vitality of pancreatic islets labeled for magnetic resonance imaging with iron particles
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DOI：
10.1016/j.transproceed.2005.09.052
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发表时间：
2005-10-01
影响因子：
0.9
通讯作者：
Saudek, F
中科院分区：
文献类型：
--
作者：
Berkova, Z;Kriz, J;Saudek, F
我们之前描述了一种使用磁共振成像，借助氧化铁超顺磁性纳米粒子（Resovist）或通过预涂抗体的磁珠（Dynabeads）进行胰岛可视化的体内方法。本研究的目的是研究胰岛标记对其质量的体外影响。将分离的大鼠胰岛与造影剂一起培养 48 小时，如果是磁性抗体包被的珠子，则仅培养 2 小时。通过静态胰岛素释放测定来测试分泌胰岛素的能力，并将结果表示为刺激指数。用碘化丙啶和吖啶橙染色以确定活细胞与死细胞的比率。 Resovist 胰岛 (n = 23) 与对照 (n = 14) 的刺激指数分别为 15.3 和 15.0，Dynabeads 胰岛 (n = 15) 与对照 (n = 12) 的刺激指数分别为 21.3 和 19.9。由活细胞/死细胞比率确定的 Resovist 胰岛与对照的活力分别为 90.8% 和 91.1% (n = 20)，而 Dynabeads 胰岛与对照的活力分别为 89.4% 和 91.8% (n = 11)。用造影剂以及带有铁珠的特异性抗体标记的胰岛不会改变体外评估的活力和胰岛素分泌能力（P>0.05）。使用铁纳米粒子的磁共振是迄今为止移植胰岛体内可视化的唯一方法。我们的数据对其临床应用做出了重要贡献。
We previously described an in vivo method for pancreatic islet visualization using magnetic resonance imaging with the aid of superparamagnetic nanoparticles of iron oxide (Resovist) or by magnetic beads precoated with antibodies (Dynabeads). The aim of this study was to investigate the in vitro effect of islet labeling on their quality. Isolated rat islets were cultivated for 48 hours with a contrast agent or, in the case of magnetic antibody-coated beads, for only 2 hours. The ability to secrete insulin was tested by a static insulin release assay and the results were expressed as a stimulation index. Staining with propidium iodide and acridine orange was performed to determine the ratio of live to dead cells. Stimulation indices in the Resovist islets (n = 23) vs controls (n = 14) were 15.3 and 15.0, respectively, and in the Dynabeads islets (n = 15) vs controls (n = 12) 21.3 and 19.9, respectively. The vitality of the Resovist islets vs controls determined by live/dead cells ratio was 90.8% and 91.1%, respectively (n = 20), and in the Dynabeads islets vs controls was 89.4% and 91.8%, respectively (n = 11). Islet labeling with the contrast agent as well as with specific antibodies with iron beads did not change the vitality and insulin-secreting capacity assessed in vitro (P >.05). Magnetic resonance using iron nanoparticles represents the only method for in vivo visualization of transplanted islets so far. Our data represent an important contribution for its clinical use.