Signal transduction pathways involved in mechanical regulation of HB-GAM expression in osteoblastic cells
Signal transduction pathways involved in mechanical regulation of HB-GAM expression in osteoblastic cells
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DOI：
10.1016/j.bbrc.2006.02.063
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发表时间：
2006-04-21
影响因子：
3.1
通讯作者：
Ignatius, A
中科院分区：
文献类型：
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作者：
Liedert, A;Kaspar, D;Ignatius, A
蛋白激酶C(PKC)、蛋白激酶A(PKA)、前列腺素合成和多种丝裂原激活的蛋白激酶(MAPK)在骨细胞中通过机械负荷被激活。我们研究了这些信号转导通路在周期性拉伸后下调成骨细胞HB-GAM表达中的作用。将这些信号转导通路的特定拮抗剂和激动剂加入到加载前的细胞和未加载的对照细胞中。用定量RT-PCR方法检测基因表达。结果表明，细胞外信号调节激酶(ERK)1/2通路、PKC、PKA、p38和C-jun氨基末端激酶MAPK参与了HB-GAM表达的机械性下调，而前列腺素的合成似乎与此无关。(C)2006 Elsevier Inc.保留所有权利。
Protein kinase C (PKC), protein kinase A (PKA), prostaglandin synthesis, and various mitogen-activated protein kinases (MAPKs) have been reported to be activated in bone cells by mechanical loading. We studied the involvement of these signal transduction pathways in the downregulation of HB-GAM expression in osteoblastic cells after cyclic stretching. Specific antagonists and agonists of these signal transduction pathways were added to cells before loading and to non-loaded control cells. Quantitative RT-PCR was used to evaluate gene expression. The data demonstrated that the extracellular signal-regulated kinase (ERK) 1/2 pathway, PKC, PKA, p38, and C-Jun N-terminal kinase MAPK participated in the mechanical downregulation of HB-GAM expression, whereas prostaglandin synthesis did not seem to be involved. (c) 2006 Elsevier Inc. All rights reserved.