Porcine CSRP3: polymorphism and association analyses with meat quality traits and comparative analyses with CSRP1 and CSRP2
Porcine CSRP3: polymorphism and association analyses with meat quality traits and comparative analyses with CSRP1 and CSRP2
复制标题
猪CSRP3：与肉质性状的多态性和关联分析以及与CSRP1和CSRP2的比较分析
DOI：
10.1007/s11033-009-9632-1
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发表时间：
2009
影响因子：
2.8
通讯作者：
Rothschild, Max F.
中科院分区：
文献类型：
--
作者：
Fan, Bin;Yuan, Fan;Xu, Xuewen;Du, Zhi-Qiang;Liu, Bang;Qiu, Haifang;Rothschild, Max F.
CRP 3是富含半胱氨酸和甘氨酸的蛋白质家族的肌肉特异性形式，在肌纤维分化中起重要作用。本研究从猪肌肉中分离并鉴定了其编码基因CSRP 3。系统发育分析表明，CSRP 3首先分化，并与其他两个成员，CSRP 1和CSRP 2区分开来。CSRP 3 mRNA在猪胚胎骨骼肌发育过程中表达上调，表明其在肌肉生长中的潜在重要性。遗传变异分析检测到多个变异，在一个约400 bp的区域覆盖外显子4及其下游内含子，并通过测序确定了两个单倍型。其中一个同义替换C1924 T用于连锁和关联分析。在伯克希尔×约克郡F2群体中，C1924 T的取代与硬度（P < 0.01）、腰肉pH、异味评分和持水力（P < 0.05）显著相关，并且对风味评分和平均糖酵解潜力有暗示性影响（P < 0.1）。关联分析结果与连锁定位和RH定位结果一致，表明该基因定位于猪染色体2 p14 -17上的一个肉质性状QTL区域。这些结果为CSRP 3作为影响猪肉品质的功能候选基因提供了基础证据。
CRP3 is the muscle-specific form of the cysteine and glycine-rich protein family and plays an important role in myofiber differentiation. Here we isolated and characterized its coding gene CSRP3 from porcine muscle. Phylogenic analyses demonstrated that CSRP3 diverged first and is distinguished from two other members, CSRP1 and CSRP2. CSRP3 mRNA was up-regulated during the development of porcine embryonic skeletal muscle, indicating its potential importance in muscle growth. Genetic variant analyses detected multiple variations in an approximately 400 bp region covering exon 4 and its downstream intron, and two haplotypes were identified by sequencing. One of synonymous substitutions C1924T was used for linkage and association analyses. It was revealed that the substitution of C1924T had significant associations with firmness (P < 0.01), Lab Loin pH, Off Flavor Score and Water Holding Capacity (P < 0.05), and a suggestive effect (P < 0.1) on Flavor Score and Average Glycolytic Potential in a Berkshire x Yorkshire F2 population. The association analyses results agreed with the gene's localization to a QTL region for meat quality traits on porcine chromosome 2p14-17 demonstrated by both linkage mapping and RH mapping. These results provide fundamental evidence for CSRP3 as a functional candidate gene affecting pig meat quality.
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DOI：
10.1152/ajpcell.1999.276.4.c900
发表时间：
1999-04
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
A. G. Schneider;Karim R. Sultan;Dirk Pette
通讯作者：
A. G. Schneider;Karim R. Sultan;Dirk Pette
影响因子：
64.5
作者：
Knöll, R;Hoshijima, M;Chien, KR
通讯作者：
Chien, KR
影响因子：
5.8
作者：
Milan, D;Hawken, R;Gellin, J
通讯作者：
Gellin, J
DOI：
10.3109/10409239309082572
发表时间：
1993
影响因子：
6.5
作者：
A. Yaron;F. Naider
通讯作者：
A. Yaron;F. Naider
影响因子：
4.1
作者：
B. D. Harper;M. Beckerle;P. Pomiès
通讯作者：
B. D. Harper;M. Beckerle;P. Pomiès