Effects of (-)-epigallocatechin gallate in liver of an animal model of combined (physical and mental) fatigue
Effects of (-)-epigallocatechin gallate in liver of an animal model of combined (physical and mental) fatigue
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DOI：
10.1016/j.nut.2008.03.001
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发表时间：
2008-06-01
期刊：
影响因子：
4.4
通讯作者：
Watanabe, Yasuyoshi
中科院分区：
文献类型：
--
作者：
Tanaka, Masaaki;Baba, Yoshitake;Watanabe, Yasuyoshi
目的：疲劳根据原因可分为身体疲劳和精神疲劳。然而，在我们的日常生活中，组合型疲劳，即身心疲劳的结合，是最常经历的。本研究评估了(-)-表没食子儿茶素没食子酸酯（EGCg）对复合疲劳的影响。方法：将大鼠置于1.5 cm高的水笼中5 d，制作复合疲劳动物模型。为了评估疲劳程度，大鼠在尾巴上绑着约为其体重8%的钢环游泳。结果：EGCg(50或100 mg/kg腹腔注射[不是25 mg/kg]) 5 d的疲劳大鼠比生理盐水的疲劳大鼠游泳时间更长。尽管血浆、脑和骨骼肌中硫代巴比妥酸反应物质的水平在对照组和疲劳大鼠之间没有差异，但疲劳动物肝脏中的硫代巴比妥酸反应物质水平高于对照组动物。口服EGCg(50或100 mg/kg) 5 d可显著降低疲劳动物肝脏中硫代巴比妥酸反应物质水平。结论：EGCg （50 mg/kg或100 mg/kg）具有明显的抗疲劳作用。口服EGCg似乎对疲劳动物氧化损伤的肝脏有抗氧化作用。(C) 2008爱思唯尔公司版权所有。
Objective: Fatigue can be classified as physical and mental depending on the cause. However, in our daily lives, combined fatigue, which is the combination of physical and mental fatigue, is most often experienced. In this study, the effects of (-)-epigallocatechin gallate (EGCg) on combined fatigue were assessed.Methods: To produce an animal model of combined fatigue, rats were kept in a cage filled with water to a height of 1.5 cm for 5 d. To evaluate the extent of fatigue, the rats swam with a load of steel rings that weighed approximately 8% of their body weight and were attached to their tails.Results: Fatigued rats treated with EGCg (50 or 100 mg/kg intraperitoneally [not for 25 mg/kg]) for 5 d could swim longer than fatigued animals given saline. Although levels of thiobarbituric acid-reactive substances in the plasma, brain, and skeletal muscle were not different between control and fatigued rats, thiobarbituric acid-reactive substance levels were higher in livers of fatigued animals than in livers of control animals. Oral intake of EGCg (50 or 100 mg/kg) for 5 d significantly decreased thiobarbituric acid-reactive substance levels in livers of fatigued animals.Conclusion: These results suggest that EGCg (50 or 100 mg/kg) is effective for attenuating fatigue. EGCg given orally appears to have an antioxidant effect on the oxidatively damaged liver of fatigued animals. (C) 2008 Elsevier Inc. All rights reserved.