The associations of gut microbiota and fecal short-chain fatty acids with bone mass were largely mediated by weight status: a cross-sectional study
The associations of gut microbiota and fecal short-chain fatty acids with bone mass were largely mediated by weight status: a cross-sectional study
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DOI：
10.1007/s00394-021-02597-x
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发表时间：
2021-06-15
影响因子：
5
通讯作者：
Zhang, Zheqing
中科院分区：
文献类型：
--
作者：
Chen, Fengyan;Wei, Qinzhi;Zhang, Zheqing
目的探讨肠道菌群和粪便短链脂肪酸（SCFAs）与6-9岁健康儿童骨量的关系。方法选择健康儿童236例，男145例，女91例。16 S rRNA基因测序用于表征其肠道微生物群的组成。采用高效液相色谱法测定粪便样品中SCFAs的总量和10种亚型。采用双能X线骨密度仪测量全身（TB）和全身减头（TBLH）的骨密度（BMD）和骨矿含量（BMC）。根据推荐参考值计算TBLH BMD的Z评分。结果在属的水平上，通过组成主成分分析，确定了4个肠道菌群主成分。在调整协变量和控制错误发现率后，多元线性回归分析显示，PC 3评分（毛梭菌属和布劳特氏菌属的阳性负荷）与TBLH BMD/BMC/Z评分、TB BMC和骨盆BMD显著负相关（β：-0.207至-0.108，p：0.002-0.048），而粪便总SCFA和几种SCFA亚型与TBLH BMD/Z评分和骨盆BMD呈正相关。（β：0.118-0.174，p：0.038-0.048）。然而，这些关联在额外调整体重后消失。中介分析表明，体重显着介导的PC 3评分和SCFAs与TBLH BMD Z评分的估计关联分别为60.4%和78.0%。结论肠道菌群组成和粪便SCFA浓度与儿童骨量的相关性主要由体重介导。
Purpose We aimed to investigate whether the gut microbiota and fecal short-chain fatty acids (SCFAs) are associated with bone mass in healthy children aged 6-9 years. Methods In this study, 236 healthy children including 145 boys and 91 girls were enrolled. 16S rRNA gene sequencing was used to characterize the composition of their gut microbiota. Total and 10 subtypes of SCFAs in the fecal samples were determined by high-performance liquid chromatography. Dual X-ray absorptiometry was used to measure the bone mineral density (BMD) and bone mineral content (BMC) for total body (TB) and total body less head (TBLH). Z score of TBLH BMD was calculated based on the recommended reference. Results Four gut microbiota principal components (PCs) were identified by the compositional principal component analysis at the genus level. After adjustment of covariates and controlling for the false discovery rate, multiple linear regression analysis showed that PC3 score (positive loadings on genera Lachnoclostridium and Blautia) was significantly negatively associated with TBLH BMD/BMC/Z score, TB BMC and pelvic BMD (beta: - 0.207 to - 0.108, p: 0.002-0.048), whereas fecal total and several subtypes of SCFAs were correlated positively with TBLH BMD/Z score and pelvic BMD (beta: 0.118-0.174, p: 0.038-0.048). However, these associations disappeared after additional adjustment for body weight. Mediation analysis suggested that body weight significantly mediated 60.4% and 78.0% of the estimated association of PC3 score and SCFAs with TBLH BMD Z score, respectively. Conclusions The associations of gut microbiota composition and fecal SCFA concentrations with bone mass in children were largely mediated by body weight.