Identification of localized redox states in plant-type two-iron ferredoxins using the nuclear Overhauser effect.
Identification of localized redox states in plant-type two-iron ferredoxins using the nuclear Overhauser effect.
复制标题
利用核奥沃豪塞效应鉴定植物型二铁铁氧还蛋白中的局部氧化还原态。
作者：
L. B. Dugad;G. L. La Mar;L. Banci;I. Bertini
利用Homeste-Overhauser效应（NOE）结合非选择性自旋-晶格弛豫测量，对钝顶螺旋藻（Spirulinaplatensis）和脐紫菜（Porphyraumbilicalis）两种典型植物型铁氧还蛋白的还原态进行了接触位移共振归属。这些结果表明，NOE应具有广泛的一般适用性的分配和电子结构的不同亚类的顺磁性铁-硫簇蛋白的阐明。NOE连接性仅在表现出与居里行为的特征性不同偏差的共振组中检测到，这为自旋哈密顿公式对反铁磁耦合铁簇的NMR性质的适用性提供了强有力的支持[Dunham，W. R.，Palmer，G.，桑兹河H、&比尔登，A. J.（1971）Biochim. Biophys. Acta 253，373-384; Banci，L.，贝尔蒂尼岛，& Luchinat，C.（1989）Struct. Bonding（出版中）]。明确鉴定了与铁（II）中心结合的两个半胱氨酸的偕β-亚甲基质子，以及与铁（III）结合的每个半胱氨酸的C α H和一个C β H。根据结合的半胱氨酸之间的偶极接触，如S的晶体坐标所预测的，鉴定与半胱氨酸41和46结合的铁为还原蛋白质中的铁（II）。platensis Fd [Tsukihara，T.，Fukuyama，K.，中村，M.，Katsube，Y.，田中，N.，Kakudo，M.，Wada，K.，Hase，T.，& Matsubara，H.（Tokyo）90，1763-1773]。（250字处删节）
The homonuclear Overhauser effect (NOE), in conjunction with nonselective spin-lattice relaxation measurements, has been employed to assign the contact-shifted resonances for the reduced form of two typical plant-type two-iron ferredoxins from the algae Spirulina platensis and Porphyra umbilicalis. These results demonstrate that the NOE should have broad general applicability for the assignments and electronic structural elucidation of diverse subclasses of paramagnetic iron-sulfur cluster proteins. NOE connectivities were detected only among sets of resonance exhibiting characteristically different deviations from Curie behavior, providing strong support for the applicability of the spin Hamiltonian formulation for the NMR properties of the antiferromagnetically coupled iron clusters [Dunham, W. R., Palmer, G., Sands, R. H., & Bearden, A. J. (1971) Biochim. Biophys. Acta 253, 373-384; Banci, L., Bertini, I., & Luchinat, C. (1989) Struct. Bonding (in press)]. The geminal beta-methylene protons for the two cysteines bound to the iron(II) center were clearly identified, as well as the C alpha H and one C beta H for each of the cysteines bound to the iron(III). The identification of the iron bound to cysteines 41 and 46 as the iron(II) in the reduced protein was effected on the basis of dipolar contacts between the bound cysteines, as predicted by crystal coordinates of S. platensis Fd [Tsukihara, T., Fukuyama, K., Nakamura, M., Katsube, Y., Tanaka, N., Kakudo, M., Wada, K., Hase, T., & Matsubara, H. (1981) J. Biochem. (Tokyo) 90, 1763-1773].(ABSTRACT TRUNCATED AT 250 WORDS)
静息状态下血红素缝隙和辣根过氧化物酶化合物 I 的核奥沃豪瑟效应研究：阳离子自由基离域至近端组氨酸的证据。
影响因子：
2.9
作者：
Thanabal,V;LaMar,GN;deRopp,JS
通讯作者：
deRopp,JS
嗜盐外硫红螺和空泡外硫红螺高电位铁硫蛋白的 Hydrogen-1 核磁共振研究：超精细位移共振的比较研究。
影响因子：
2.9
作者：
Krishnamoorthi,R;Markley,JL;Cusanovich,MA;Przysiecki,CT;Meyer,TE
通讯作者：
Meyer,TE