Characterization of a New Stearoyl-acyl Carrier Protein Desaturase Gene from Jatropha curcas
Characterization of a New Stearoyl-acyl Carrier Protein Desaturase Gene from Jatropha curcas
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DOI：
10.1007/s10529-006-0034-3
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发表时间：
2006-05
影响因子：
2.7
通讯作者：
L. Tong;S. Peng;Wu-yuan Deng;Dan-Wei Ma;Xu Ying;X. Meng;Chen Fang
中科院分区：
文献类型：
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作者：
L. Tong;S. Peng;Wu-yuan Deng;Dan-Wei Ma;Xu Ying;X. Meng;Chen Fang
利用RT-PCR和RACE技术从麻疯树发育种子中获得了一个新的硬脂酰酰基载体蛋白去饱和酶全长cDNA。序列比对表明，所得氨基酸序列与其他硬脂酰酰基载体蛋白去饱和酶具有较高的相似性。该基因功能表达为inE。在添加菠菜铁氧化还蛋白的情况下，体外检测重组蛋白的去饱和活性。Southern blot分析表明该基因是一个小基因家族的成员。Northern blot分析显示，该基因在红枣发育果实中高度表达。curcas。
A new full-length cDNA of stearoyl-acyl carrier protein desaturase was obtained by RT-PCR and RACE techniques from developing seeds ofJatropha curcas. Sequence alignment showed that its deduced amino acid sequence had high similarity with other stearoyl-acyl carrier protein desaturases. The gene was functionally expressed inE. coliand the desaturating activity of recombinant protein was easily detected when assayedin vitrowith added spinach ferredoxin. Southern blot analysis indicated that the gene was a member of a small gene family. Northern blot analysis revealed it was highly expressed in developing fruits ofJ. curcas.