Solution-based circuits enable rapid and multiplexed pathogen detection
Solution-based circuits enable rapid and multiplexed pathogen detection
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DOI：
10.1038/ncomms3001
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发表时间：
2013-06-01
影响因子：
16.6
通讯作者：
Kelley, Shana O.
中科院分区：
文献类型：
--
作者：
Lam, Brian;Das, Jagotamoy;Kelley, Shana O.
疾病标志物的电子读数在检测临床样本中的小组生物标志物方面提供了令人信服的简单性、敏感性和特异性；然而，最重要的新兴疾病检测，如传染病物种形成和抗生素耐药性分析，将需要询问样本中的许多生物标志物。由于难以在廉价的平台上处理基于电极的大型传感器阵列，大型标记板的电子读数一直受到阻碍。在这里，我们报告了一个新的概念-基于解决方案的电路形成的芯片，使高度多路电化学传感在无源芯片上可行。基于解决方案的电路切换携带信息的信号读出通道，并消除来自相邻生物分子特异性微传感器的所有可测量串扰。我们构建了具有这一进步的芯片，并证明它们成功地分析了未纯化的样品，并准确地分类了临床相关浓度的病原体。我们还表明，在样品引入2分钟后，可以准确地读取特征分子。
Electronic readout of markers of disease provides compelling simplicity, sensitivity and specificity in the detection of small panels of biomarkers in clinical samples; however, the most important emerging tests for disease, such as infectious disease speciation and antibiotic-resistance profiling, will need to interrogate samples for many dozens of biomarkers. Electronic readout of large panels of markers has been hampered by the difficulty of addressing large arrays of electrode-based sensors on inexpensive platforms. Here we report a new concept-solution-based circuits formed on chip-that makes highly multiplexed electrochemical sensing feasible on passive chips. The solution-based circuits switch the information-carrying signal readout channels and eliminate all measurable crosstalk from adjacent, biomolecule-specific microsensors. We build chips that feature this advance and prove that they analyse unpurified samples successfully, and accurately classify pathogens at clinically relevant concentrations. We also show that signature molecules can be accurately read 2 minutes after sample introduction.