Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles.
Novel expression of coat proteins from thermophilic bacteriophage ΦIN93 and evaluation for assembly into virus-like particles.
复制标题
DOI：
10.1016/j.pep.2021.105932
复制
发表时间：
2021-11
影响因子：
1.6
通讯作者：
Tumban E
中科院分区：
文献类型：
--
作者：
Zhai L;Anderson D;Bruckner E;Tumban E
病毒样颗粒（vlp）有可能被用作展示平台，以开发针对传染性和非传染性病原体的疫苗。然而，大多数用作疫苗展示平台的vlp来自感染人类的病毒；不幸的是，大多数人已经存在针对这些平台的抗体，因此，这些疫苗的免疫原性可能会受到损害。从感染细菌（噬菌体）的病毒衍生的VLP平台，特别是感染细菌的噬菌体，不定植人类，不太可能在人群中预先存在针对平台的抗体。在这项研究中，我们评估了来自嗜热噬菌体（ΦIN93）的两种假定的外壳蛋白（ORF13和OFR14）是否可以从嗜热细菌（如大肠杆菌）中表达和纯化。我们还评估了表达的外壳蛋白是否可以组装形成VLPs。截断的ORF13和OFR14在细菌中成功共表达；共表达的截断蛋白形成了看起来像VLPs的椭圆形结构，但它们的大小比真正的ΦIN93病毒小。
Virus-like particles (VLPs) have the potential to be used as display platforms to develop vaccines against infectious and non-infectious agents. However, most VLPs used as vaccine display platforms are derived from viruses that infect humans; unfortunately, most humans already have pre-existing antibodies against these platforms and thus, the immunogenicity of these vaccines may be compromised. VLP platforms derived from viruses that infect bacteria (bacteriophages), especially bacteriophages that infect bacteria, which do not colonize humans are less likely to have pre-existing antibodies against the platforms in the human population. In this study, we assessed whether two putative coat proteins (ORF13 and OFR14) derived from a thermophilic bacteriophage (ΦIN93) can be expressed and purified from a mesophilic bacterium such as E. coli. We also assessed whether expressed coat proteins can assemble to form VLPs. Truncated versions of ORF13 and OFR14 were successfully co-expressed in bacteria; the co-expressed truncated proteins formed oval structures that look like VLPs, but their sizes were less than those of an authentic ΦIN93 virus.
登录
查看更多内容
影响因子：
2.7
作者：
Ong HK;Tan WS;Ho KL
通讯作者：
Ho KL
影响因子：
4.8
作者：
Gerlich WH
通讯作者：
Gerlich WH
影响因子：
13.8
作者：
Wang, YL;Geer, LY;Bryant, SH
通讯作者：
Bryant, SH
影响因子：
4.8
作者：
Knuchel, Marlyse C.;Marty, Rene R.;Naim, Hussein Y.
通讯作者：
Naim, Hussein Y.
影响因子：
6.2
作者：
Tyler M;Tumban E;Chackerian B
通讯作者：
Chackerian B