Comparative study of the quercetin, ascorbic acid, glutathione and superoxide dismutase for nitric oxide protecting effects in mouse gastric fundus
Comparative study of the quercetin, ascorbic acid, glutathione and superoxide dismutase for nitric oxide protecting effects in mouse gastric fundus
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DOI：
10.1016/j.ejphar.2012.10.009
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发表时间：
2013-01-05
影响因子：
5
通讯作者：
Ogulener, Nuran
中科院分区：
文献类型：
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作者：
Ertug, Peyman U.;Aydinoglu, Fatma;Ogulener, Nuran
比较槲皮素、Cu/Zn超氧化物歧化酶（Cu/Zn SOD）、抗坏血酸和谷胱甘肽对一氧化氮（NO）诱导的小鼠胃底舒张的预防作用。此外，研究了槲皮素对组织总氧化剂和抗氧化剂水平的影响。氮能刺激（4 Hz，25 V，0.1ms，10 s-train）和外源性NO（10 p，M）引起舒张。邻苯三酚（10 μ M），氢醌（100 μ M）和LY 83583（6-苯胺基-喹啉-5，8-醌，5 μ M）抑制氮能松弛。用邻苯三酚、氢醌和LY 83583观察到的抑制被槲皮素（0.1 μ M）阻止。此外，抗坏血酸（500 μ M），谷胱甘肽（100 μ M）和Cu/Zn SOD（100 U/ml）防止抑制作用的超氧阴离子发生器上的放松氮刺激和NO。二乙基二硫代氨基甲酸（DETCA; 8 mM）抑制氮能松弛。槲皮素、抗坏血酸、谷胱甘肽或Cu/Zn SOD可阻断DETCA对一氧化氮能刺激的抑制作用和NO诱导的舒张作用。DETCA与邻苯三酚、对苯二酚或LY 83583联用可增强对舒张的抑制作用。槲皮素、抗坏血酸和谷胱甘肽预处理可阻断DETCA和LY-83583对NO和NO的抑制作用，但Cu/Zn SOD不能阻断DETCA和LY-83583的抑制作用。此外，槲皮素增加组织总抗氧化能力，降低组织氧化水平和氧化应激指数在DETCA治疗组。这些结果表明，槲皮素具有抗氧化作用，能保护NO免受内源性超氧阴离子的破坏，并增强其生物活性，提示槲皮素可能通过Cu/Zn SOD、谷胱甘肽或抗坏血酸还原的方式清除超氧阴离子。(c)2012爱思唯尔有限公司版权所有。
The aim of this work was to compare the preventing capacity of quercetin with Cu/Zn superoxide dismutase (Cu/Zn SOD), ascorbic acid and glutathione on nitric oxide (NO)-induced relaxation in mouse gastric fundus. Furthermore, the effects of the quercetin on the tissue level of total oxidant and antioxidant was investigated. Nitrergic stimulation (4 Hz, 25 V, 0.1 ms, 10 s-train) and exogenous NO (10 p,M) induced relaxation. Pyrogallol (10 mu M), hydroquinone (100 mu M) and LY83583 (6-Anilino-quinolin-5,8-quinone, 5 mu M) inhibited nitrergic relaxations. The inhibition observed with pyrogallol, hydroquinone and LY83583 was prevented by quercetin (0.1 mu M). Also, ascorbic acid (500 mu M), glutathione (100 mu M) and Cu/Zn SOD (100 U/ml) prevented the inhibitory effect of superoxide anion generators on the relaxation to nitrergic stimulation and NO. Diethyldithiocarbamic acid (DETCA; 8 mM) inhibited nitrergic relaxations. DETCA-induced inhibition on nitrergic stimulation and NO-induced relaxation was prevented by quercetin, ascorbic acid, glutathione or Cu/Zn SOD. DETCA plus pyrogallol, hydroquinone or LY83583 strengthened the inhibition on the relaxations. Also, pre-treatment with quercetin, ascorbic acid and glutathione prevented the inhibitory effect of DETCA plus LY-83583 on the relaxation to nitrergic stimulation and NO but Cu/Zn SOD did not prevent this inhibition. Also, quercetin increased tissue total antioxidant capacity and decreased tissue oxidant level and oxidative stress index in DETCA-treatment group. These results indicate that quercetin has antioxidant effect and protects NO from endogenous superoxide anion-driven inactivation and enhances its biological activity, suggesting that quercetin may scavenge superoxide anion in a Cu/Zn SOD, glutathione or ascorbic acid-inhibitable manner. (c) 2012 Elsevier B.V. All rights reserved.