Cytotoxicity testing of silver-containing burn treatments using primary and immortal skin cells
Cytotoxicity testing of silver-containing burn treatments using primary and immortal skin cells
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DOI：
10.1016/j.burns.2014.02.009
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发表时间：
2014-12-01
期刊：
影响因子：
2.7
通讯作者：
Cuttle, Leila
中科院分区：
文献类型：
--
作者：
Boonkaew, Benjawan;Kempf, Margit;Cuttle, Leila
先前已经开发了一种新型烧伤伤口水凝胶敷料，其由2-丙烯酰胺基-2-甲基丙磺酸钠盐与银纳米颗粒（银AMPS）组成。本研究比较了该敷料与市售银产品的细胞毒性; Acticoat（TM）、PolyMem银（R）和Flamazine（TM）乳膏。将人角质形成细胞（HaCaT和原代HEK）和正常人成纤维细胞（NHF）暴露于在Nunc（TM）聚碳酸酯插入物上孵育的敷料24、48和72小时。进行四种不同的细胞毒性测定，包括台盼蓝细胞计数、MTT、Celltiter-Blue（TM）和甲苯胺蓝表面积测定。结果表示为与未处理对照相比的相对细胞活力。Acticoat（TM）和Flamazine（TM）乳膏的细胞毒性作用取决于暴露时间和细胞类型。暴露24小时后，Acticoat（TM）和Flamazine（TM）乳膏对所有测试的细胞系都是有毒的。令人惊讶的是，用Acticoat（TM）和Flamazine（TM）处理的HaCaTs在48和72小时具有改善的存活能力，而HEK和NHF在任何处理下的存活率都没有改善。新型银水凝胶和PolyMem银在每个时间间隔对所有测试的细胞系显示出低细胞毒性，并且这些结果支持使用新型银水凝胶作为烧伤伤口敷料的可能性。依赖HaCaT细胞作为准确的角质形成细胞模型的研究人员应该意识到它们对初级皮肤细胞的反应不同。(C)2014 Elsevier Ltd和ISBI。All rights reserved.
A novel burn wound hydrogel dressing has been previously developed which is composed of 2-acrylamido-2-methylpropane sulfonic acid sodium salt with silver nanoparticles (silver AMPS). This study compared the cytotoxicity of this dressing to the commercially available silver products; Acticoat (TM), PolyMem Silver (R) and Flamazine (TM) cream. Human keratinocytes (HaCaT and primary HEK) and normal human fibroblasts (NHF) were exposed to dressings incubated on Nunc (TM) polycarbonate inserts for 24, 48 and 72 h. Four different cytotoxicity assays were performed including; Trypan Blue cell count, MTT, Celltiter-Blue (TM) and Toluidine Blue surface area assays. The results were expressed as relative cell viability compared to an untreated control. The cytotoxic effects of Acticoat (TM) and Flamazine (TM) cream were dependent on exposure time and cell type. After 24 h exposure, Acticoat (TM) and Flamazine (TM) cream were toxic to all tested cell lines. Surprisingly, HaCaTs treated with Acticoat (TM) and Flamazine (TM) had an improved ability to survive at 48 and 72 h while HEKs and NHFs had no improvement in survival with any treatment. The novel silver hydrogel and PolyMem Silver (R) showed low cytotoxicity to all tested cell lines at every time interval and these results support the possibility of using the novel silver hydrogel as a burn wound dressing. Researchers who rely on HaCaT cells as an accurate keratinocyte model should be aware that they can respond differently to primary skin cells. (C) 2014 Elsevier Ltd and ISBI. All rights reserved.