Bisamides from Aglaia species: structure analysis and potential to reverse drug resistance with cultured cells.
Bisamides from Aglaia species: structure analysis and potential to reverse drug resistance with cultured cells.
复制标题
来自米香属物种的双酰胺：结构分析和逆转培养细胞耐药性的潜力。
DOI：
10.1021/np50094a004
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发表时间：
1993
影响因子：
5.1
通讯作者：
Pezzuto,JM
中科院分区：
文献类型：
--
作者：
Saifah,E;Puripattanavong,J;Likhitwitayawuid,K;Cordell,GA;Chai,H;Pezzuto,JM
通过广泛的核磁共振研究，包括homomorphicCOSY，NOESY，APT，HETCOR，和选择性INEPT技术确定米仔兰。对梨形生物碱的~（13）C-NMR归属进行了修订。通过几个2D NMR光谱（homopolycoSY、NOESY和HETCOR）的检查实现了pirifera的分离，并通过选择性INEPT NMR实验证实。在11个人类癌细胞系中进行了两种生物碱的细胞毒性潜力的评估，沿着的还有来自米仔兰的另外两种双酰胺，odorine [3]和5 '-^/-odorine [4]。这些双酰胺均未显示出显著的细胞毒性。然而，梨形碱[2]、气味碱[3]和5 '-^/-气味碱[4]被发现通过增强长春碱的抗癌活性来抑制长春碱抗性KB细胞的生长。肉桂酸衍生的双酰胺是在米仔兰属（楝科）植物中发现的一组独特的生物碱（1^ 4）。最近已经从米仔兰（Aglaia pirifera Hance）中分离出了梨形碱[2]（1），而从米仔兰（Aglaia odorata Lour）和米仔兰（Aglaia roxburghiana）中鉴定出了气味碱[3]和5 '-β-气味碱[4]（2，3）·关于（-）-odorinol [5}在体内P-388系统中的抗白血病活性的最新报告（4）促使我们对这种结构类型内的其他双酰胺的抗癌潜力的研究产生兴趣，作为我们正在进行的从植物中寻找抗癌剂的一部分。在我们的植物化学
Aglaia pyramidata, was determined through extensive nmr studies, including homonuclear COSY, NOESY, APT, HETCOR, and selective INEPT techniques. Revision of the 13C-nmr assignment of piriforme [2], an alkaloid previously isolated from A. pirifera, was achieved by examination of several 2D nmr spectra (homonuclear COSY, NOESY, and HETCOR) and con-firmed by selective INEPT nmr experiments. Evaluation of the cytotoxic potentialof the two al-kaloids, along with two other bisamides from Aglaia odorata, odorine [3] and 5'-^/-odorine [4], was carried out in eleven human cancer cell lines. None of these bisamides showed significant cytotoxicity. Nevertheless, piriforme [2], odorine [3], and 5'-^/-odorine [4] were found to in-hibit the growthof the vinblastine-resistant KB cells by enhancing the anticancer activity of vinblastine.Cinnamic acid-derived bisamides are a unique group of alkaloids found in plants of the genus Aglaia (Meliaceae)(1^ 4). Piriferine [2] has been recently isolated from Aglaia pirifera Hance (1), whereas odorine [3] and 5'-^/-odorine [4] were identified from Ag-laia odorata Lour, and Aglaia roxburghiana (2, 3)· A recent report on the antileukemic ac-tivity of (—)-odorinol [5} in the in vivo P-388 system (4) prompted our interest in the investigation of anticancer potential of other bisamides within this structural type, as a part of our ongoing search for anticancer agents from plants. During our phytochemical