Effect of sodium hydrosulphide after acute compression injury of spinal cord
Effect of sodium hydrosulphide after acute compression injury of spinal cord
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DOI：
10.1016/j.brainres.2013.06.023
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发表时间：
2013-08-21
期刊：
影响因子：
2.9
通讯作者：
Agrawal, S. K.
中科院分区：
文献类型：
--
作者：
Kesherwani, V.;Nelson, K. S.;Agrawal, S. K.
背景：脊髓白色损伤的早期治疗已被发现是有益的。目的：研究NaHS对脊髓白色物质夹压损伤的保护作用。方法：在8-10周龄Wistar大鼠中，通过在T8-T8暴露脊髓来诱导损伤。在T10节段，通过椎板切除术并应用35 g夹子1 min。在损伤1 h后腹腔内给予50 AM NaHS。0.5 mm脊髓组织收集损伤后8小时，从两侧，包括震中和背柱显微解剖和用于进一步study.Results：NaHS治疗减少一氧化氮（NO）的27%和脂质过氧化物（LPO）的18%相比，损伤，这是衰减氧化应激的标志。免疫印迹显示髓过氧化物酶（MPO）水平显著变化，下降了10%。GSH含量治疗组较损伤组增加44%。NaHS处理使Nrf-2表达增加1.8倍。我们发现NaHS处理降低了GFAP的表达8%，NF-200在处理后没有显著变化，H和E染色没有明显的形态学变化。结论：根据上述数据，我们得出结论，NaHS在50 μ M剂量损伤后1小时，通过增加Nrf-2的表达，降低了损伤部位的NO，LPO，GFAP和MPO水平。我们期望在脊髓损伤急性期这些参数的降低将有助于神经保护和再生。(C)2013爱思唯尔有限公司版权所有。
Background: Early treatment of spinal cord white matter injury has been found beneficial. H2S, a neurotransmitter is neuroprotective at lower doses.Purpose: In the present study the effect of NaHS after clip compression injury of spinal cord white matter in vivo was studied.Methods: The injury was induced in 8-10 weeks old Wistar rats by exposing the spinal cord at T8-T10 level by laminectomy and applying 35 g clip for 1 min. A dose of 50 AM NaHS was given intraperitoneally after 1 h of injury. 0.5 mm Spinal cord tissues were collected 8 h after injury from both sides including epicenter and dorsal column was microdissected and used for further study.Results: NaHS treatment decreases nitric oxide (NO) by 27% and lipid peroxide (LPO) by 18% as compared to injury, which are hallmark of attenuation in oxidative stress. Western blots shows significant changes in Myeloperoxidase (MPO) level went down by 10%. GSH contents increased 44% in treated group as compared to the injury group. NaHS treatment increased Nrf-2 expression 1.8 times. We found NaHS treatment reduced the GFAP expression 8%, there was no significant changes in NF-200 after treatment and no evident morphological changes with H and E staining.Conclusions: With the above data we conclude that NaHS at 50 mu M dose at 1 h after injury reduces the NO, LPO, GFAP and MPO level at injury site by increasing the expression of Nrf-2. We expect that a decrease in these parameters during acute phase of spinal cord injury would be helpful in neuroprotection and regeneration. (C) 2013 Elsevier B.V. All rights reserved.