Separation of peptides on the basis of the difference in positive charge: simultaneous isolation of C-terminal and blocked N-terminal peptides from tryptic digests.
Separation of peptides on the basis of the difference in positive charge: simultaneous isolation of C-terminal and blocked N-terminal peptides from tryptic digests.
复制标题
根据正电荷差异分离肽：从胰蛋白酶消化物中同时分离 C 端和封闭的 N 端肽。
DOI：
--
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Koichi Suzuki
中科院分区：
文献类型：
--
作者：
H. Kawasaki;S. Imajoh;Koichi Suzuki
用去肌红蛋白和钙调蛋白的胰酶消化，考察了基于正电荷差异的微尺度多肽分离。通过这种分离方法，可以在不进行任何化学修饰的情况下，将C-末端和封闭的N-末端多肽选择性地分离到同一组分中。分离的多肽，包括内源多肽，通过反相高效液相色谱进一步纯化，纯化后的多肽可以直接进行序列和氨基酸分析。用该方法成功地分离了钙激活中性蛋白酶的N-末端多肽。
The microscale separation of peptides based on the difference in positive charge was examined with tryptic digests of apomyoglobin and calmodulin. By this separation method, C-terminal and blocked N-terminal peptides could be selectively isolated in the same fraction without any chemical modifications. Separated peptides, including internal peptides, were further purified by reversed phase high performance liquid chromatography, and the purified peptides could be directly subjected to sequence and amino acid analyses. The N-terminal peptides of calcium-activated neutral protease were successfully isolated by this method.