A direct estimate of the human αβ T cell receptor diversity
A direct estimate of the human αβ T cell receptor diversity
复制标题
DOI：
10.1126/science.286.5441.958
复制
发表时间：
1999-10-29
期刊：
影响因子：
56.9
通讯作者：
Kourilsky, P
中科院分区：
文献类型：
--
作者：
Arstila, TP;Casrouge, A;Kourilsky, P
T细胞抗原受体的有效曲目的产生和维护对于免疫系统至关重要，但是人体中估计的10（12）个T细胞表达的不同T细胞受体（TCR）的数量尚不清楚。在这项研究中，TCR基因的扩增和测序表明，血液中大约有10（6）个不同的β链，每种配对平均，至少有25种不同的α链。在记忆子集中，多样性降低到1 x 10（5）至2 x 10（5）不同的β链，每个β链仅与单个alpha链配对。因此，幼稚的曲目高度多样，而在这里，记忆室的三分之一占T细胞种群的贡献不到总多样性的1％。
Generation and maintenance of an effective repertoire of T cell antigen receptors are essential to the immune system, yet the number of distinct T cell receptors (TCRs) expressed by the estimated 10(12) T cells in the human body is not known. In this study, TCR gene amplification and sequencing showed that there are about 10(6) different beta chains in the blood, each pairing, on the average, with at Least 25 different alpha chains. In the memory subset, the diversity decreased to 1 x 10(5) to 2 x 10(5) different beta chains, each pairing with only a single alpha chain. Thus, the naive repertoire is highly diverse, whereas the memory compartment, here one-third of the T cell population, contributes Less than 1 percent of the total diversity.