Inflammatory biomarkers and growth factors in saliva and gingival crevicular fluid of e-cigarette users, cigarette smokers, and dual smokers: A pilot study.
Inflammatory biomarkers and growth factors in saliva and gingival crevicular fluid of e-cigarette users, cigarette smokers, and dual smokers: A pilot study.
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电子烟使用者、吸烟者和双重吸烟者唾液和龈沟液中的炎症生物标志物和生长因子：一项试点研究。
DOI：
10.1002/jper.19-0457
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发表时间：
2020-10
影响因子：
4.3
通讯作者：
Rahman, Irfan
中科院分区：
文献类型：
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作者：
Ye, Dongxia;Gajendra, Sangeeta;Lawyer, Gina;Jadeja, Neelam;Pishey, Deepa;Pathagunti, Srinivasa;Lyons, Janet;Veazie, Peter;Watson, Gene;McIntosh, Scott;Rahman, Irfan
吸烟仍然是全球主要的公共卫生威胁之一。电子烟（e-cigs）提供了传统吸烟的替代方案；然而，使用电子烟的风险和益处的证据基础是新的且不断增长。在这项横断面试点研究中，评估了电子烟的使用对唾液和龈沟液（GCF）生物特征的影响，并与吸烟者（CS）、双重使用者和非使用者的特征进行了比较。还评估了电子烟使用者（EC）和其他群体之间的全身炎症介质。这项试点横断面研究招募了由四组组成的志愿者参与者：非吸烟者 (NS)、CS、EC 以及双重 EC 和吸烟者 (DS)。收集唾液和 GCF 样本，并通过免疫测定（酶联免疫吸附测定和 Luminex）分析炎症、氧化应激、抗炎脂质介质、组织损伤和修复以及生长因子的生物标志物。通过唾液可替宁确认吸烟状况。与EC和DS组相比，CS组前列腺素E2水平显着升高，但与非吸烟者（NS）相比，EC和DS组没有显着差异。 EC 和 NS（髓过氧化物酶 [MPO]、基质金属蛋白酶-9）组之间以及 DS 和 EC 之间炎症介质生物标志物（晚期糖基化终产物受体 [RAGE]、MPO、子宫珠蛋白/CC-10）之间观察到统计学显着差异； DS 和 NS 组之间的细胞外新鉴定的 RAGE 结合蛋白以及 CS 和 NS 组之间的 MPO。免疫（S100A8、S100A9、半乳糖凝集素-3）、组织损伤和修复（Serpine1/PAI-1）和生长因子（脑源性神经营养因子、成纤维细胞生长因子、血小板源性生长因子-AA、血管内皮生长因子等）生物标志物在各组之间均未发现统计学显着差异。不同吸烟状况组之间的可测量健康结果存在统计学上的显着差异，这表明吸烟/电子烟对口腔健康产生不同的影响。
Cigarette smoking remains one of the leading public health threats worldwide. Electronic cigarettes (e-cigs) provide an alternative to conventional cigarette smoking; however, the evidence base of risks and benefits of e-cig use is new and growing. In this cross-sectional pilot study, the effect of e-cig use on biological profiles in saliva and gingival crevicular fluid (GCF) was assessed and compared with the profiles of cigarette smokers (CS), dual users, and non-users. The systemic inflammatory mediators between e-cig users (EC) and these other groups were also assessed. This pilot cross-sectional study recruited volunteer participants consisting of four groups, non-smokers (NS), CS, EC, and dual EC and cigarette smokers (DS). Saliva and GCF samples were collected and analyzed for biomarkers of inflammation, oxidative stress, anti-inflammatory lipid mediators, tissue injury and repair, and growth factors with immunoassay (enzyme-linked immunosorbent assay and Luminex). Smoking status was confirmed via salivary cotinine. Prostaglandin E2 level was significantly increased in CS compared with EC and DS, but not significantly different in EC and DS groups compared with non-smokers (NS). Statistically significant differences were observed between groups of EC and NS (myeloperoxidase [MPO], matrix metalloproteinase-9) as well as between DS and EC for biomarkers of inflammatory mediators (receptor for advanced glycation end products [RAGE], MPO, uteroglobin/CC-10); between groups of DS and NS for extracellular newly identified RAGE binding protein and between CS and NS for MPO. No statistically significant differences in biomarkers of immunity (S100A8, S100A9, galectin-3), tissue injury and repair (Serpine1/PAI-1) and growth factors (brain-derived neurotrophic factor, fibroblast growth factors, platelet-derived growth factor-AA, vascular endothelial growth factor, and others) were found between any of groups. Statistically significant differences in measurable health outcomes were found between different smoking status groups, suggesting that smoking/vaping produces differential effects on oral health.