Immunosuppressive effect of a new antibiotic: ovalicin.
Immunosuppressive effect of a new antibiotic: ovalicin.
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一种新抗生素的免疫抑制作用：卵黄素。
DOI：
10.1159/000386780
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
H. Stähelin
中科院分区：
文献类型：
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作者：
S. Lazàry;H. Stähelin
材料和方法动物实验。小鼠：白化和(白化×dba/2)F₁小鼠，2~3月龄，雌雄不拘。大鼠：两个月龄雄性Wistar大鼠。抗原，免疫。每只小鼠静脉注射10%洗净的绵羊红细胞悬浮液0.5ml。对于EAE，抗原是整个大鼠脊髓，在改良的弗氏佐剂中乳化：脊髓2.5g，生理盐水2.2ml，Arlacel A 0.9ml，Nujol 5.4ml和55 mg，干草分枝杆菌灭活。大鼠每只后肢皮内注射0.1毫升该乳剂。
Materials and MethodsAnimals. Mice: Albino and (albino× DBA/2) F₁ mice, two to three months of age of both sexes. Rats: two months old male Wistar rats. Antigens, immunisation. 0.5 ml of a 10% suspension of washed sheep red blood cells in saline was administered intravenously to each mouse.For EAE, the antigen was whole rat spinal cord, emulsified in a modified Freund's adjuvant: 2.5 g spinal cord, 2.2 ml saline, 0.9 ml Arlacel A, 5.4 ml Nujol and 55 mg killed, dried Mycobacterium phlei. Rats received 0.1 ml of this emulsion intradermally in each hind